B95a

B-lymphoblastoid, EBV-transformed

B95a is a derivative of the cell line B95-8 and was adapted to growth in adherence to the substrate surface. The parent line B95-8 was derived from a cotton-top tamarin (Saguinas oedipus). The B-lymphoblastoid cells are transformed with Epstein-Barr virus (EBV). B95a has been reported to be 10,000-fold more sensitive to measles virus present in clinical specimens than Vero cells and are useful host cells for the isolation of measles virus.

NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA.

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442) (for growth medium) or 2% FBS / FCS (F2442) (for maintenance medium).

Split sub-confluent cultures (70-80%) 1:3 every 3-4 days using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C; cells grow initially as a monolayer, but will pile up and detach in maintenance medium.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.