MRC-5 PD 19 Cell Line human
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 5072101, human lung, Fibroblast-like
别名:
MRC-V Cells, MRC5 Cells, Medical Research Council Cell Strain-5
生物来源
human lung
描述
Human fetal lung
包装
tube of 5 μg 05072101-DNA-5UG
pkg of vial of cells 05072101-1VL
生长模式
Adherent
核型
2n=46, diploid
形态学
Fibroblast-like
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
It has been used as a cell substrate to study the production of recombinant MVA (modified vaccinia virus Ankara).
生化/生理作用
Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.
Human foetal lung
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15
制备说明
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C.
其他说明
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法规信息
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