biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 11060815-DNA-5UG, pkg of vial of cells 11060815-1VL
ImmunoDon
DA rat spleen
Myeloma
Y3.AG.1.2.3
Reactivity
Human HLA B
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
-140 to -196°C
Immunogen
Human peripheral blood mononuclear cells
Biochem/physiol Actions
Monoclonal antibody human leukocyte antigen B (HLA-b)
Rat hybridoma producing antibody against human HLA B. Cambridge Enterprise Reference number AB74:www.enterprise.cam.ac.uk.
Preparation Note
IDMEM (I3390) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin (T4424) or mechanical agitation maybe required to dislodge them from the plastic.
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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