产品名称
Vero C1008 [Vero 76, clone E6, Vero E6], NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85020206, African green monkey kidney, Epithelial
biological source
African green monkey kidney
description
Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
packaging
tube of 5 μg 85020206-DNA-5UG
pkg of vial of cells 85020206-1VL
growth mode
Adherent
karyotype
2n = 60
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
VERO C1008 has been used for transfection, cultivation and propagation of West Nile virus, yellow fever virus, ZIKA, Ebola virus, Sabethes flavivirus (SbFV), Crimean-Congo Haemorrhagic Fever (CCHF), Human-derived Lake Victoria Marburg virus strain Popp.
Biochem/physiol Actions
A subclone of Vero76 (Sigma catalog no. 85020205) exhibiting a similar range of virus susceptibility (haemorrhagic viruses). They show some degree of contact inhibition and are suitable for supporting the growth of slowly replicating viruses.
Monkey African Green kidney
General description
Vero cells are derived from the kidney of an African green monkey. These are anchorage-dependent cells that have applications in molecular and cell biology research. Vero E6 cells enable achieving high titers of severe acute respiratory syndrome coronavirus (SARS-CoV virus) and are also used in the cultivation of Rabies virus, Reovirus and Japanese encephalitis virus.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
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