LS40 human cell line

Consanguineous: No

Homozygous: No

HLA DRB1: 404
HLA DRB4: 103
HLA DPB1: 0201 0301

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

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HLA A: 1 26
HLA C: 2 3
HLA B: 27 60
Bw4/bw6: 4 6
HLA Dw: 14
HLA DR: 4
HLA DR53: 53
HLA DQ: 3
HLA DP: 2

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.