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Merck
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COS-7 Cell Line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87021302, African green monkey kidney, Fibroblast

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UNSPSC代码:
41106514
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生物来源

African green monkey kidney

描述

Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

包装

tube of 5 μg 87021302-DNA-5UG
pkg of vial of cells 87021302-1VL

生长模式

Adherent

核型

Not specified

形态学

Fibroblast

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

应用

COS-7 Cell Line has been used to:
  • test the functional role of ankyrin B in ouabain/Na,K-ATPase/1,4,5-trisphosphate receptor (IP3R) signaling
  • study the expression of green fluorescent protein-tagged constructs of neuropathy target esterase (NTE) and mutant proteins lacking the transmembrane, catalytic and regulatory domains
  • study the distribution of peroxisomes
Virus replication studies: SV40

生化/生理作用

Derived from CV-1 simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be handled at laboratory containment level 2.
Monkey African green kidney, SV40 transformed

制备说明

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)..
Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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