COS-7 Cell Line

COS-7 Cell Line has been used to:

• test the functional role of ankyrin B in ouabain/Na,K-ATPase/1,4,5-trisphosphate receptor (IP3R) signaling
• study the expression of green fluorescent protein-tagged constructs of neuropathy target esterase (NTE) and mutant proteins lacking the transmembrane, catalytic and regulatory domains
• study the distribution of peroxisomes

Virus replication studies: SV40

Derived from CV-1 simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be handled at laboratory containment level 2.

Monkey African green kidney, SV40 transformed

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)..

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.