生物来源
African green monkey kidney
描述
Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
包装
tube of 5 μg 87021302-DNA-5UG
pkg of vial of cells 87021302-1VL
生长模式
Adherent
核型
Not specified
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
细胞系来源
Monkey African green kidney, SV40 transformed
细胞系描述
Derived from CV-1 simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be handled at laboratory containment level 2.
应用
COS-7 Cell Line has been used to:
- test the functional role of ankyrin B in ouabain/Na,K-ATPase/1,4,5-trisphosphate receptor (IP3R) signaling
- study the expression of green fluorescent protein-tagged constructs of neuropathy target esterase (NTE) and mutant proteins lacking the transmembrane, catalytic and regulatory domains
- study the distribution of peroxisomes
Virus replication studies: SV40
培养基
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
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