biological source
human blood
packaging
tube of 5 μg 88052052-DNA-5UG, pkg of vial of cells 88052052-1VL
ethnicity
Amish
gender
male
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
-140 to -196°C
General description
Consanguineous: Yes
Biochem/physiol Actions
A: 201
Cw: 602
B: 5701
HLA DRA: 101
HLA DRB1: 701
HLA DRB4: 0103102N
HLA DQAP: 2.1
HLA DQA1: 201
HLA DQB1: 3032
HLA DPA1: 1
HLA DPB1: 401
Cw: 602
B: 5701
HLA DRA: 101
HLA DRB1: 701
HLA DRB4: 0103102N
HLA DQAP: 2.1
HLA DQA1: 201
HLA DQB1: 3032
HLA DPA1: 1
HLA DPB1: 401
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
Preparation Note
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
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HLA A: 2
HLA A ief: 2.2
HLA C: 6
HLA B: 57
HLA B ief: 57.2
Bw4/bw6: 4
HLA Dw: 11
HLA DR: 7
HLA DQ: 9
HLA DP: 4
HLA A ief: 2.2
HLA C: 6
HLA B: 57
HLA B ief: 57.2
Bw4/bw6: 4
HLA Dw: 11
HLA DR: 7
HLA DQ: 9
HLA DP: 4
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.