KT14 human cell line

Consanguineous: No

Homozygous: No

A: 2402 2602
Cw: 0801 1402
B: 5101 4006
HLA DRB1: 9
HLA DQA1: 3
HLA DQB1: 03
HLA DPB1: 02012 1901

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

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HLA A: 24 26
HLA A ief: 24.1 26*
HLA C: 8
HLA B: 51 61
HLA B ief: 51 61
Bw4/bw6: 4 6
HLA Dw: 23
HLA DR: 9
HLA DR53: 53
HLA DQ: 9
HLA DP: 2 -

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.