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UNSPSC Code:
41106514
Biological source:
human blood
Technique(s):
cell culture | mammalian: suitable
Ethnicity:
Caucasian
Gender:
female
Growth mode:
Suspension
Morphology:
Lymphoblastoid
biological source
human blood
packaging
tube of 5 μg 94031663-DNA-5UG, pkg of vial of cells 94031663-1VL
ethnicity
Caucasian
gender
female
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
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Biochem/physiol Actions
Not specified
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
Preparation Note
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
HLA A: 24 26
HLA C: 6 0
HLA B: 13 41
HLA DR: 12 7
HLA DQ: 3 2
HLA C: 6 0
HLA B: 13 41
HLA DR: 12 7
HLA DQ: 3 2
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
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