biological source
human blood
manufacturer/tradename
ECACC
packaging
tube of 5 μg 94050311-DNA-5UG, pkg of vial of cells 94050311-1VL
ethnicity
French Canadian
gender
female
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
-140 to -196°C
General description
Consanguineous: No
Homozygous: No
Biochem/physiol Actions
A: 03 25
B: 47 37
HLA DRB1: 0101 1417
HLA DRB3: 0101
HLA DQA1: 0101 0103
HLA DQB1: 0501 0603
HLA DPA1: 1
HLA DPB1: 401
B: 47 37
HLA DRB1: 0101 1417
HLA DRB3: 0101
HLA DQA1: 0101 0103
HLA DQB1: 0501 0603
HLA DPA1: 1
HLA DPB1: 401
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
Preparation Note
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
HLA A: 25 3
HLA C: 6
HLA B: 37 47
HLA DR: 1 6
HLA DR52: 52
HLA DQ: 1
HLA C: 6
HLA B: 37 47
HLA DR: 1 6
HLA DR52: 52
HLA DQ: 1
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Only the genomic DNA extracted from the cell line is available for purchase by commercial organizations, and not the cell line itself. No licensing agreement is required when the DNA extracted from this cell line are used for internal research purposes only. Frozen cell cultures can be supplied only to academic and not-for-profit organisations. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding other authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.