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WIL-JON human cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

别名:

DO208915

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关于此项目

UNSPSC Code:
41106514
Biological source:
human blood
Technique(s):
cell culture | mammalian: suitable
Ethnicity:
Australian Caucasian
Gender:
female
Growth mode:
Suspension
Morphology:
Lymphoblastoid

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biological source

human blood

packaging

tube of 5 μg 95010407-DNA-5UG, pkg of vial of cells 95010407-1VL

ethnicity

Australian Caucasian

gender

female

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

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General description

Consanguineous: No

Biochem/physiol Actions

A: 2501
Cw: 1203
B: 1801
HLA DRA: 102
HLA DRB1: 1501
HLA DRB5: 101
HLA DRB6: 201
HLA DQA1: 102
HLA DQB1: 0602 0603
HLA DPA1: 1
HLA DPB1: 02012 2301
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Preparation Note

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
HLA A: 25
HLA A ief: 25
HLA B: 18
HLA B ief: 18.1
Bw4/bw6: 6
HLA Dw: 2
HLA DR: 15
HLA DR51: 51
HLA DQ: 6
HLA DP: 2 4

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

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