SPACH human cell line

Consanguineous: Yes

Homozygous: Yes

A: 3101
Cw: 102
B: 1501
HLA DRA: 102
HLA DRB1: 8021
HLA DQA1: 401
HLA DQB1: 402
HLA DPA1: 1
HLA DPB1: 402

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

HLA A: 31
HLA A ief: 31
HLA C: 1
HLA B: 62
HLA B ief: 62.3
Bw4/bw6: 6
HLA Dw: 8.2
HLA DR: 8
HLA DQ: 4
HLA DP: 4