D1152
杜氏改良 Eagle 培养基 - 高葡萄糖
HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture
别名:
DME, DMEM
产品名称
杜氏改良 Eagle 培养基 - 高葡萄糖, HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Application
Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。
Preparation Note
按照每升培养基17.3克粉末配制。
补充3.7 g/L碳酸氢钠。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源培养基
此项目有
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实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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