D2906
DMEM/F-12
With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture
别名:
DME/F-12, 1:1混合
产品名称
杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture
质量水平
表单
powder
技术
cell culture | mammalian: suitable
组分
NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM
运输
ambient
储存温度
2-8°C
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一般描述
Dulbecco改良Eagle培养基/Ham F-12营养混合物适合低血清条件下广泛的细胞类型生长。克隆和干细胞培养的研究者通常更喜欢去除酚红。
应用
Dulbecco改良Eagle培养基/Ham F-12营养混合物已用于培养：
- Hep-2细胞
- 小鼠成纤维L‐细胞
- L‐ Wnt家族成员3A(Wnt3a)细胞
- 原代水牛胚胎成纤维细胞(BEF)
制备说明
并补充1.2 g/L碳酸氢钠。
配方中每升培养基含有15.6克的粉末。
储存分类代码
11 - Combustible Solids
WGK
WGK 1
法规信息
动植物来源培养基
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Tao Fu et al.
FEBS letters, 593(15), 2040-2050 (2019-06-04)
Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family, which functions in embryo implantation and decidualization. The expression, function and regulation of Osm in mouse uteri during early pregnancy remain unknown. We show that Osm is mainly expressed
Nereo Kalebic et al.
EMBO reports, 17(3), 338-348 (2016-01-14)
We have applied the CRISPR/Cas9 system in vivo to disrupt gene expression in neural stem cells in the developing mammalian brain. Two days after in utero electroporation of a single plasmid encoding Cas9 and an appropriate guide RNA (gRNA) into
Eric M Erkenbrack et al.
PLoS biology, 16(8), e2005594-e2005594 (2018-08-25)
Among animal species, cell types vary greatly in terms of number and kind. The number of cell types found within an organism differs considerably between species, and cell type diversity is a significant contributor to differences in organismal structure and
C Zhang et al.
Osteoarthritis and cartilage, 26(6), 824-833 (2018-03-20)
Adipokines play roles in the pathogenesis of osteoarthritis (OA). Fatty acid binding protein 4 (FABP4) is a novel adipokine that is closely associated with obesity and metabolic diseases. The aim of this study was to discover the potential role of
Peter S B Finnie et al.
Current biology : CB, 30(10), 1934-1940 (2020-04-04)
Memory allows organisms to predict future events based on their prior sampling of the world. Rather than faithfully encoding each detail of related episodes, the brain is thought to incrementally construct probabilistic estimates of environmental statistics that are re-evaluated each
商品
Nutrient Mixture F-12 Ham Formulation
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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