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质量水平
检测方案
≥96%
形式
powder, crystals or chunks
技术
titration: 96-104% (with NaOH)
颜色
white to light brown, and Faint Red and Faint Grey to Light Grey
mp
280 °C
溶解性
H2O: 50 mg/mL
应用
diagnostic assay manufacturing
hematology
histology
储存温度
−20°C
SMILES字符串
O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2
InChI key
DXWSCXIZIHILNP-UHFFFAOYSA-N
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一般描述
应用
生化/生理作用
警示用语:
Danger
危险分类
Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
What is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), soluble in?
DAB HCl is soluble in water at 50 mg/ml yielding a clear to slightly hazy solution. For consistency, it is important to allow DAB to come to room temperature before making solutions. Water quality is extremely important, so only high quality deionized or distilled water should be used to make DAB solutions.
What is the solution stability of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
In our experience, solutions of DAB should be made immediately prior to use for best results. If and only if necessary, solutions can be kept in the dark and refrigerated for overnight storage. Based on our Immunology Department’s experience, storing the solution overnight did not give good reproducibility. However, the literature indicates that it is possible to store solutions of DAB at -20°C. DAB dissolved in Tris buffered saline (pH 7.6) at 0.6 mg/ml was reported to be stable for several weeks at 4°C.
Can the solutions of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), be filtered?
DAB solution may be filtered through a 0.2 micron filter immediately prior to use.
Do you have a staining protocol for Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
Dissolve 10 mg in 15 mL of Tris buffered saline, pH 7.6 (Tris-buffered saline tablet, Sigma Product No. T5030 may be used). Add 12 uL of fresh 30% hydrogen peroxide (Sigma Product No. H1009) prior to use. Solution may be filtered through a 0.2 micron filter immediately prior to use if necessary. DAB and hydrogen peroxide concentrations may be adjusted to suit individual applications. The stain can be enhanced with Ni. Make up 0.3% (w/v) NiCl2 in water. Add 1 mL of the NiCl2 stock to 9 mL DAB solution.
What is the shelf life of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
This product has a shelf life of 3 years.
How is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), shipped and stored?
The product is shipped at ambient temperature. However, it is stored at -20 degrees C for long term storage.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。
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