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Merck
CN

D5671

DMEM - high glucose

With sodium Bicarbonate, without ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

别名:

DME, DMEM

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UNSPSC Code:
12352207
NACRES:
NA.75

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产品名称

杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
glucose: high
NaHCO3: yes
sodium pyruvate: no
phenol red: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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Application

Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。

General description

这种DMEM高葡萄糖培养基是一种不含添加因子（常见修改）如L-丙氨酰-L-谷氨酰胺、HEPES或丙酮酸钠的1x完全培养基。 它与原始DMEM高葡萄糖配方的不同点在于用吡哆醇代替了吡哆醛。 吡哆醛是一种不稳定的培养基成分。 该培养基需要添加L-谷氨酰胺或于L-丙氨酰-L-谷氨酰胺。

Preparation Note

添加0.584 g/L L-谷氨酰胺。

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动植物来源培养基
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBM0550
RNBM0069
RNBL8843
RNBL7075
RNBL6563

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Smita Krishnaswamy et al.
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Cellular regulatory networks are not static, but continuously reconfigure in response to stimuli via alterations in protein abundance and confirmation. However, typical computational approaches treat them as static interaction networks derived from a single time point. Here, we provide methods
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Frontiers in cellular and infection microbiology, 7, 452-452 (2017-12-07)
Resistance to anthelmintic drugs is a major problem in the global fight against parasitic nematodes infecting humans and animals. While previous studies have identified mutations in drug target genes in resistant parasites, changes in the expression levels of both targets
Massiel Cepeda-Molero et al.
PLoS pathogens, 13(10), e1006706-e1006706 (2017-10-31)
Enteropathogenic E. coli (EPEC) is a human pathogen that causes acute and chronic pediatric diarrhea. The hallmark of EPEC infection is the formation of attaching and effacing (A/E) lesions in the intestinal epithelium. Formation of A/E lesions is mediated by
Anna Kobuszewska et al.
SLAS technology, 22(5), 536-546 (2017-04-22)
Lab-on-a-chip systems are increasingly used as tools for cultures and investigation of cardiac cells. In this article, we present how the geometry of microsystems and microenvironmental conditions (static and perfusion) influence the proliferation, morphology, and alignment of cardiac cells (rat
Debora Lia et al.
Journal of cell science, 131(12) (2018-06-01)
Accumulation of 8-oxoguanine (8-oxoG) in mitochondrial DNA and mitochondrial dysfunction have been observed in cells deficient for the DNA glycosylase OGG1 when exposed to oxidative stress. In human cells, up to eight mRNAs for OGG1 can be generated by alternative
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