D6171
DMEM - high glucose
HEPES modification, with sodium bicarbonate, without ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
别名:
DME, DMEM
产品名称
杜氏改良 Eagle 培养基 - 高葡萄糖, HEPES modification, With 4500 mg/L glucose, 25 mM HEPES, and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
sodium pyruvate: no
HEPES: 25 mM
glucose: high
NaHCO3: yes
L-glutamine: no
phenol red: yes
运输
ambient
储存温度
2-8°C
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应用
Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。
制备说明
添加0.584 g/L L-谷氨酰胺。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
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