D6546
DMEM - high glucose
With sodium Bicarbonate, sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
别名:
DME, DMEM
产品名称
杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high
运输
ambient
储存温度
2-8°C
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一般描述
这种DMEM高葡萄糖培养基是一种添加了丙酮酸钠的1x完全培养基。 它还与原始DMEM高葡萄糖配方的不同点在于用吡哆醇代替了吡哆醛。 吡哆醛是一种不稳定的培养基成分。 该培养基需要添加L-谷氨酰胺或于L-丙氨酰-L-谷氨酰胺。
应用
Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。
制备说明
添加0.584 g/L L-谷氨酰胺。
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
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商品
Cell culture protocol for passaging and splitting suspension cell lines
用于传代和分装悬浮细胞系的细胞培养方案
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