Merck
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文件

D6665

Sigma-Aldrich

2′,7′-二氯荧光素

suitable for use as an indicator in chloride titration, ~90% (TLC), powder

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别名:
二氯荧光素；荧光素27
经验公式（希尔记法）:
C20H10Cl2O5
CAS号:
76-54-0
分子量:
401.20
Beilstein:
58009
EC 号:
200-968-6
MDL编号:
MFCD00005047
PubChem化学物质编号:
24894041
NACRES:
NA.47

质量水平

200

检测方案

~90% (TLC)

形式

powder

mp

280 °C (dec.) (lit.)

溶解性

ethanol: 25 mg/mL

密度

0.79 g/cm3

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

VFNKZQNIXUFLBC-UHFFFAOYSA-N

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相关类别

一般描述

2′,7′-二氢荧光素是一种氧化敏感荧光探针。2′7′-二氯荧光素二乙酸酯在活性氧（活性氧）存在下氧化成2′7′-二氯荧光素。引用

应用

2 & # 8242；，7 & # 8242；-二氯荧光素已被用作氧化敏感荧光探针来测量细胞中活性氧（活性氧）的形成。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What can be used to make a solution of 2´,7´-dichlorofluorescein?

    2´,7´-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2´,7´-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.

  6. In what types of applications can 2´,7´-Dichlorofluorescein be used?

    Some applications for use of 2´,7´-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC.  See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2´,7´-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a  fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).

  7. What excitation /emission wavelengths are best for fluorescence detection of 2´,7´-Dichlorofluorescein?

    2´,7´-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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