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D7295

脱氧核苷酸混合物，10mM

Molecular Biology Reagent

别名:

脱氧核苷酸混合物，10mM

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关于此项目

NACRES:
NA.52
UNSPSC Code:
41106305
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
liquid
Solubility:
H2O: soluble at 20 °C
Storage temp.:
−20°C
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biological source

synthetic (organic)

Quality Level

300

assay

≥99%

form

liquid

concentration

10 mM

color

colorless

solubility

H2O: soluble at 20 °C

application(s)

agriculture

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

相关类别

General description

核苷酸是有机分子，可作为核酸（如DNA和RNA）的单体或亚基。核苷酸是核酸的结构单元，由一个含氮碱基（嘌呤或嘧啶）、一个五碳糖（核糖或脱氧核糖）和至少一个磷酸基团组成。一个核苷与一个磷酸基团组合形成一个核苷酸。脱氧核苷酸混合物是一种便捷式预混dNTP溶液，以高质量分子生物学级水为溶剂，含有等摩尔（10mM）的UltraPure超纯dATP、dCTP、dGTP和TTP钠盐。1 µL足够进行一次标准50 µL PCR反应。

Application

dNTP混合物已用于：

  • PCR扩增昆虫、 真菌、病毒、人类来源分离的基因组DNA
  • 总RNA逆转录为cDNA
  • 作为聚合酶链式反应(PCR)DNA扩增混合物组分
  • 常规和长片段PCR
  • 手动和自动DNA测序
  • cDNA合成和标记反应

Features and Benefits

  • 每种dNTP的纯度：最低99%
  • 方便配制；每50 μL PCR使用1 μL
  • 等摩尔量的各种dNTP可减少移液步骤
  • 最大限度地降低PCR污染风险
  • UltraPure超纯dNTP可帮助最大限度地提高关键PCR反应的一致性和得率

存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
WXBF8209V
WXBF8015V
WXBF8461V
WXBF7757V
WXBF7547V

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Abril Sánchez-Botet et al.
Scientific reports, 8(1), 11797-11797 (2018-08-09)
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Cancer investigation, 24(5), 484-491 (2006-08-31)
BRCA1 and BRCA2 gene mutations in patients with breast and/or ovarian cancer have been not characterized in the Turkish population until now. A total of 87 female subjects from two sets of families (38 families total) provided blood samples from
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ShenYanXiaoBai granules is a traditional Chinese herbal medicine, It is used widely for the treatment of proteinuria caused by various kidney diseases. This study investigated the mechanism of Shenyan Xiaobai Granule in the treatment of nephritis proteinuria. 100 male wistar
Ayokunle O Olanrewaju et al.
ACS sensors, 5(4), 952-959 (2020-04-07)
Poor adherence to pre-exposure prophylaxis (PrEP) and antiretroviral therapy (ART) can lead to human immunodeficiency virus (HIV) acquisition and emergence of drug-resistant infections, respectively. Measurement of antiviral drug levels provides objective adherence information that may help prevent adverse health outcomes.
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实验方案

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

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全球贸易项目编号

货号GTIN
D7295-5ML04061838104953
D7295-.5ML04061838104946
D7295-.2ML04061838104939
D7295-.25ML04061838056863
D7295-20X.2ML04061838087355

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