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D8764

脱氧核糖核酸酶II 来源于牛脾脏

Name: 脱氧核糖核酸酶II 来源于牛脾脏
Brand: SIGMA

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

别名:

DNase II, 脱氧核苷酸酶 3′-寡核苷酸脱氢酶

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关于此项目

化学文摘社编号:

9025-64-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-801-8

MDL number:

MFCD00130919

EC 号:

3.1.22.1 (BRENDA, IUBMB)

Specific activity:

≥1,000 units/mg protein

Biological source:

bovine spleen

Concentration:

≥50% protein

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biological source

bovine spleen

Quality Level

200

type

Type V

form

essentially salt-free, lyophilized powder

specific activity

≥1,000 units/mg protein

mol wt

38 kDa

concentration

≥50% protein

technique(s)

DNA extraction: suitable, tissue culture: suitable

suitability

suitable for molecular biology

application(s)

cell analysis

foreign activity

RNase ≤0.1%

storage temp.

−20°C

Application

牛脾脱氧核糖核酸酶Ⅱ已用于：

作为从结肠样品分离免疫细胞的消化混合液组分
消化视网膜外植体以制备单细胞悬液
分离人结肠组织的基质细胞
作为从大鼠心脏制备胚胎心肌细胞的解离培养基组分

Biochem/physiol Actions

脱氧核糖核酸酶II，也称为酸性DNA酶，可水解天然和变性DNA中的脱氧核糖核苷酸连接键，产生带有3′-磷酸的产物。体外最佳pH范围是4.5-5.0。它还可在pH 5.6-5.9下作用于对硝基苯基磷酸二酯。分子量约为38,000 Da。

Other Notes

在 pH 4.6 25℃条件下，一个Kunitz单位可每分钟在每毫升内产生0.001的ΔA₂₆₀；[Mg²⁺] = 0.83 mM

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料

动植物源性产品

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历史批次信息供参考:

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Dopamine D2 receptor stimulation differentially affects voltage-activated calcium channels in rat pituitary melanotropic cells.

Keja J A, et al.

The Journal of Physiology, 450(1), 409-435 (1992)

Cardiomyocyte-transistor-hybrids for sensor application.

S Ingebrandt et al.

Biosensors & bioelectronics, 16(7-8), 565-570 (2001-09-07)

An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described

[Partial purification and properties of deoxyribonucleases from eggs and liver of Xenopus laevis. Comparison with deoxyribonuclease II from bovine spleen].

K Nitta et al.

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 114(2), 119-128 (1994-02-01)

Deoxyribonucleases from eggs and the liver of Xenopus laevis were partially purified by DEAE-cellulose and heparin-Sepharose affinity column chromatographies. The fractions having egg and liver DNase activities were eluted on high performance liquid chromatography through TSK gel G3000SW at the

Protective targeting of high mobility group box chromosomal protein 1 in a spontaneous arthritis model.

Therese Ostberg et al.

Arthritis and rheumatism, 62(10), 2963-2972 (2010-06-10)

High mobility group box chromosomal protein 1 (HMGB-1) is a DNA binding nuclear protein that can be released from dying cells and activated myeloid cells. Extracellularly, HMGB-1 promotes inflammation. Clinical and experimental studies demonstrate that HMGB-1 is a pathogenic factor

[Nucleic acid and protein synthesis of splenic lymphocytes of the rat under the influence of mucopolysaccharide-polysulfuric acid-esters (author's transl)].

K Tempel

Arzneimittel-Forschung, 30(7), 1119-1123 (1980-01-01)

The influence of three mucopolysaccharide-polysulfuric acid-esters (MPS) of different molecular weight on DNAase II-activity as well as on nucleic acid and protein synthesis of lymphocytes of rat spleen has been investigated in vitro. The results are summarized as follows: 1.

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全球贸易项目编号

货号	GTIN
D8764-150KU	04061832755489
D8764-300KU	04061832755496
D8764-30KU	04061832755502

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