D8764
脱氧核糖核酸酶II 来源于牛脾脏
Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
别名:
DNase II, 脱氧核苷酸酶 3′-寡核苷酸脱氢酶
生物来源
bovine spleen
质量水平
类型
Type V
表单
essentially salt-free, lyophilized powder
比活
≥1,000 units/mg protein
分子量
38 kDa
浓度
≥50% protein
技术
DNA extraction: suitable
tissue culture: suitable
适用性
suitable for molecular biology
应用
cell analysis
异质活性
RNase ≤0.1%
储存温度
−20°C
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应用
牛脾脱氧核糖核酸酶Ⅱ已用于：
- 作为从结肠样品分离免疫细胞的消化混合液组分
- 消化视网膜外植体以制备单细胞悬液
- 分离人结肠组织的基质细胞
- 作为从大鼠心脏制备胚胎心肌细胞的解离培养基组分
生化/生理作用
脱氧核糖核酸酶II，也称为酸性DNA酶，可水解天然和变性DNA中的脱氧核糖核苷酸连接键，产生带有3′-磷酸的产物。体外最佳pH范围是4.5-5.0。它还可在pH 5.6-5.9下作用于对硝基苯基磷酸二酯。分子量约为38,000 Da。
其他说明
在 pH 4.6 25℃条件下，一个Kunitz单位可每分钟在每毫升内产生0.001的ΔA260；[Mg2+] = 0.83 mM
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
低风险生物材料
动植物源性产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
K Tempel
Arzneimittel-Forschung, 30(7), 1119-1123 (1980-01-01)
The influence of three mucopolysaccharide-polysulfuric acid-esters (MPS) of different molecular weight on DNAase II-activity as well as on nucleic acid and protein synthesis of lymphocytes of rat spleen has been investigated in vitro. The results are summarized as follows: 1.
STUDIES ON ACID DEOXYRIBONUCLEASE. II. ISOLATION AND CHARACTERIZATION OF SPLEEN-ACID DEOXYRIBONUCLEASE.
G BERNARDI et al.
Biochemistry, 3, 1419-1426 (1964-10-01)
S Ingebrandt et al.
Biosensors & bioelectronics, 16(7-8), 565-570 (2001-09-07)
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described
K Nitta et al.
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 114(2), 119-128 (1994-02-01)
Deoxyribonucleases from eggs and the liver of Xenopus laevis were partially purified by DEAE-cellulose and heparin-Sepharose affinity column chromatographies. The fractions having egg and liver DNase activities were eluted on high performance liquid chromatography through TSK gel G3000SW at the
Therese Ostberg et al.
Arthritis and rheumatism, 62(10), 2963-2972 (2010-06-10)
High mobility group box chromosomal protein 1 (HMGB-1) is a DNA binding nuclear protein that can be released from dying cells and activated myeloid cells. Extracellularly, HMGB-1 promotes inflammation. Clinical and experimental studies demonstrate that HMGB-1 is a pathogenic factor
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