D8764
脱氧核糖核酸酶II 来源于牛脾脏
Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
别名:
DNase II, 脱氧核苷酸酶 3′-寡核苷酸脱氢酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-801-8
MDL number:
Specific activity:
≥1,000 units/mg protein
Biological source:
bovine spleen
Concentration:
≥50% protein
产品名称
脱氧核糖核酸酶II 来源于牛脾脏, Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
biological source
bovine spleen
type
Type V
form
essentially salt-free, lyophilized powder
specific activity
≥1,000 units/mg protein
mol wt
38 kDa
concentration
≥50% protein
technique(s)
DNA extraction: suitable
tissue culture: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
foreign activity
RNase ≤0.1%
storage temp.
−20°C
Quality Level
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Application
牛脾脱氧核糖核酸酶Ⅱ已用于：
- 作为从结肠样品分离免疫细胞的消化混合液组分
- 消化视网膜外植体以制备单细胞悬液
- 分离人结肠组织的基质细胞
- 作为从大鼠心脏制备胚胎心肌细胞的解离培养基组分
Biochem/physiol Actions
脱氧核糖核酸酶II，也称为酸性DNA酶，可水解天然和变性DNA中的脱氧核糖核苷酸连接键，产生带有3′-磷酸的产物。体外最佳pH范围是4.5-5.0。它还可在pH 5.6-5.9下作用于对硝基苯基磷酸二酯。分子量约为38,000 Da。
Other Notes
在 pH 4.6 25℃条件下，一个Kunitz单位可每分钟在每毫升内产生0.001的ΔA260；[Mg2+] = 0.83 mM
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
动植物源性产品
此项目有
S Ingebrandt et al.
Biosensors & bioelectronics, 16(7-8), 565-570 (2001-09-07)
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described
K Nitta et al.
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 114(2), 119-128 (1994-02-01)
Deoxyribonucleases from eggs and the liver of Xenopus laevis were partially purified by DEAE-cellulose and heparin-Sepharose affinity column chromatographies. The fractions having egg and liver DNase activities were eluted on high performance liquid chromatography through TSK gel G3000SW at the
STUDIES ON ACID DEOXYRIBONUCLEASE. II. ISOLATION AND CHARACTERIZATION OF SPLEEN-ACID DEOXYRIBONUCLEASE.
G BERNARDI et al.
Biochemistry, 3, 1419-1426 (1964-10-01)
Dopamine D2 receptor stimulation differentially affects voltage-activated calcium channels in rat pituitary melanotropic cells.
Keja J A, et al.
The Journal of Physiology, 450(1), 409-435 (1992)
K Tempel
Arzneimittel-Forschung, 30(7), 1119-1123 (1980-01-01)
The influence of three mucopolysaccharide-polysulfuric acid-esters (MPS) of different molecular weight on DNAase II-activity as well as on nucleic acid and protein synthesis of lymphocytes of rat spleen has been investigated in vitro. The results are summarized as follows: 1.
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