Merck
CN
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安全信息

D9443

SAFC

杜氏改良 Eagle 培养基-低葡萄糖

liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling in cell culture applications

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别名:
DME, DMEM
MDL编号:
MFCD00217342
NACRES:
NA.75

描述

With 1000mg/L D-glucose, L-glutamine, and sodium bicarbonate. Without arginine, leucine, lysine, sodium pyruvate, and phenol red

质量水平

500

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

NaHCO3: 3.7 g/L
sodium pyruvate: no
glucose: 1.0 g/L
L-glutamine: 0.584 g/L
phenol red: no
HEPES: no

运输

ambient

储存温度

2-8°C

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相关类别

重悬

设计用于细胞培养应用中氨基酸稳定同位素标记。
补充 0.100 g/L L -精氨酸- 13 C 6 、 15 N 4 HCl（产品 608033）、0.100 g/L L -亮氨酸- 13 C 6 、 15 N（产品 608068）和 0.100 g/L L -赖氨酸- 13 C 6 、 15 N 2 （产品 608041）。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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A M Edick et al.
Journal of dairy science, 104(1), 1123-1135 (2020-11-10)
The objective of this study was to determine the role of GCN2 in the response to AA deprivation of primary bovine mammary epithelial cells (BMEC). Cells were isolated from the mammary tissue of 2 lactating Holstein cows by enzymatic digestion
Mahesh Saqcena et al.
PloS one, 8(8), e74157-e74157 (2013-08-27)
In multicellular organisms, cell division is regulated by growth factors (GFs). In the absence of GFs, cells exit the cell cycle at a site in G1 referred to as the restriction point (R) and enter a state of quiescence known
Dipsikha Biswas et al.
Cell death discovery, 7(1), 241-241 (2021-09-17)
Triple-negative breast cancers (TNBCs) are characterized by poor survival, prognosis, and gradual resistance to cytotoxic chemotherapeutics, like doxorubicin (DOX). The clinical utility of DOX is limited by its cardiotoxic and chemoresistant effects that manifest over time. To induce chemoresistance, TNBC
Sona Hubackova et al.
Molecular oncology, 13(7), 1467-1489 (2019-03-29)
Radiation and chemotherapy represent standard-of-care cancer treatments. However, most patients eventually experience tumour recurrence, treatment failure and metastatic dissemination with fatal consequences. To elucidate the molecular mechanisms of resistance to radio- and chemotherapy, we exposed human cancer cell lines (HeLa
Dipsikha Biswas et al.
The Journal of biological chemistry, 295(46), 15597-15621 (2020-09-04)
Branched-chain α-keto acids (BCKAs) are catabolites of branched-chain amino acids (BCAAs). Intracellular BCKAs are cleared by branched-chain ketoacid dehydrogenase (BCKDH), which is sensitive to inhibitory phosphorylation by BCKD kinase (BCKDK). Accumulation of BCKAs is an indicator of defective BCAA catabolism
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