Merck
CN

E2129

Sigma-Aldrich

伊文思蓝

Dye content ≥75 %

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别名:
Direct Blue 53
经验公式(希尔记法):
C34H24N6Na4O14S4
CAS号:
分子量:
960.81
颜色索引号:
23860
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.47

形式

powder

质量水平

组成

Dye content, ≥75%

application(s)

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1\N=N\c2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(\N=N\c5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;

InChI key

ATNOAWAQFYGAOY-GPTZEZBUSA-J

Gene Information

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一般描述

伊文思蓝(EB)C.I.23860是一种合成双偶氮染料,也称为T-1824和直接蓝53。水溶性较高的EB染料对血清白蛋白具有很强的亲和力,是一种高分子量蛋白质示踪剂。

应用

伊文思蓝是一种著名的重要染色剂,适用于组织学和荧光显微镜。伊文思蓝已用于检查细胞活力和研究血脑屏障(BBB)的渗透性。其他应用包括展示特定的垂体细胞类型、中性粒细胞质膜的选择性染色以及在荧光显微镜中作为一种猝灭剂抑制不必要的自体荧光。

生化/生理作用

伊文思蓝是一种非渗透染料。在质膜存在损伤时,染料可进入细胞质和核中并将它们染成蓝色。伊文思蓝可用于检查细胞的活力。它还可用于研究BBB(血脑屏障)的通透性。染料与白蛋白结合,从而表明BBB已分解为蛋白。

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Carc. 1B

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

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  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should Product E2129, Evans Blue, be dissolved?

    This product is soluble in water, alcohol, acids and alkalies.

  4. What is the solution stability of Product E2129, Evans Blue?

    This product is rather stable in aqueous solution and may be autoclaved at 15 psi for 30 min. However, dye made up in physiological saline should not be autoclaved.

  5. What is the difference between Evans Blue Products E2129 and 206334?

    Both products are Evans Blue. Product No. E2129 has a dye content of 80% and Product No. 206334 has a dye content of approximately 85%.

  6. What is the shelf life of Product E2129, Evans Blue?

    This product has a retest date of 4 years.  This information can be found on the Certificate of Analysis.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Can Evans Blue, Product E2129, be used as a counterstain for FITC?

    This product can be used as a counterstain in immunohistochemistry when using FITC. After staining for immunofluorescence, dip sections in a 0.1% (w/v) in water solution of Evans Blue for 5-10 minutes. Rinse well in fresh PBS or water before coverslipping. Reference: Immunocytochemistry, Theory and Practice, p. 82 (1988).

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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