E2906
抗-dEGF受体，细胞外结构域抗体，小鼠单克隆
clone C-273, purified from hybridoma cell culture
别名:
抗-果蝇 表皮生长因子受体, 抗-dEGFR
登录 查看组织和合同定价。
选择尺寸
关于此项目
Conjugate:
unconjugated
Clone:
C-273, monoclonal
Application:
immunohistochemistry
indirect ELISA
western blot
indirect ELISA
western blot
Species reactivity:
Drosophila
Citations:
15
Technique(s):
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using recombinant protein representing amino acids 299-359 of the Drosophila EGFR extracellular domain
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using recombinant protein representing amino acids 299-359 of the Drosophila EGFR extracellular domain
产品名称
抗-dEGF受体，细胞外结构域抗体，小鼠单克隆, clone C-273, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
C-273, monoclonal
form
buffered aqueous solution
species reactivity
Drosophila
concentration
~1.2 mg/mL
technique(s)
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using recombinant protein representing amino acids 299-359 of the Drosophila EGFR extracellular domain
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
Drosophila melanogaster ... Egfr(37455)
Application
适用于以下方法：
- 免疫组织化学
- 间接ELISA
- 使用代表果蝇EGFR细胞外结构域的氨基酸299-359的重组蛋白以0.25-0.5μg/mL的浓度进行蛋白质印迹分析
Biochem/physiol Actions
果蝇表皮生长因子受体（EGFR）参与许多发育过程，例如：卵极性，腹外胚层中的细胞识别，神经发生，马氏管的发育以及幼虫眼和翼的发育。被果蝇EGF受体激活的下游信号分子包括：Src同源性（Shc），DRK（哺乳动物Grb2的同源物），被DRK激活的鸟嘌呤核苷酸交换因子（SOS）以及下游靶标，包括Ras，Raf和Rolled的直系同源物（MAP激酶）。
表皮生长因子受体（EGFR）与其同源配体结合，该配体会导致受体酪氨酸激酶的自磷酸化，并随后激活参与调节细胞增殖、分化和存活的信号转导途径。EGFR在肿瘤细胞系中过表达，并与不良预后相关，导致存活率降低。它的激活在肿瘤细胞中对化疗和放射治疗的抵抗中也起作用。EGFR酪氨酸激酶抑制剂（TKIs）可能作为晚期肺腺癌的新治疗选择。其配体在果蝇CNS中起作用并维持神经胶质存活。果蝇EGF受体在果蝇视网膜发育过程中起关键作用。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
抗dEGF受体，细胞外结构域抗体，小鼠单克隆（小鼠IgG1同种型）衍生自杂交瘤C-273，该杂交瘤通过小鼠骨髓瘤细胞（NS1细胞）和来自用在果蝇表皮生长因子受体（EGFR）的细胞外部分编码的重组蛋白免疫的BALB/c小鼠的脾细胞融合产生。果蝇EGF受体，又称Torpedo或DER，有四个配体：Gurken，Spitz（主要配体），Vein和Argos。两种辅助蛋白调节其信号传导：菱形和星形。
Physical form
溶于含有15 mM叠氮化钠的0.01 M磷酸盐缓冲液中。
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Nicole T Revaitis et al.
Development (Cambridge, England), 147(15) (2020-07-19)
To bridge the gap between qualitative and quantitative analyses of the epidermal growth factor receptor (EGFR) in tissues, we generated an sfGFP-tagged EGF receptor (EGFR-sfGFP) in Drosophila The homozygous fly appears similar to wild type with EGFR expression and activation
Regulating the dynamics of EGF receptor signaling in space and time
Shilo BZ
Development, 132(18), 4017-4027 (2005)
Dan Liu et al.
Nature communications, 13(1), 6243-6243 (2022-10-22)
Cell competition is a conserved homeostatic mechanism whereby epithelial cells eliminate neighbors with lower fitness. Cell communication at the interface of wild-type "winner" cells and polarity-deficient (scrib-/-) "losers" is established through Sas-mediated Ptp10D activation in polarity-deficient cells. This tumor-suppressive cell
Roy S Herbst
International journal of radiation oncology, biology, physics, 59(2 Suppl), 21-26 (2004-05-15)
The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein that constitutes one of four members of the erbB family of tyrosine kinase receptors. Binding of EGFR to its cognate ligands leads to autophosphorylation of receptor tyrosine kinase and subsequent
Josefa Steinhauer et al.
Journal of cell science, 126(Pt 19), 4469-4478 (2013-08-02)
Epidermal growth factor receptor (EGFR) ligands undergo a complex series of processing events during their maturation to active signaling proteins. Like its mammalian homologs, the predominant Drosophila EGFR ligand Spitz is produced as a transmembrane pro-protein. In the secretory pathway
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持