Merck
CN

E5134

Sigma-Aldrich

乙二胺四乙酸 二钠盐 二水合物

suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

登录查看公司和协议定价

别名:
EDTA 二钠盐, EDTA-Na2, 乙二胺四乙酸Na2, 乙二胺四乙酸盐 二钠盐 二水合物, 二钠乙二胺四乙酸盐 二水合物, 依地酸钙钠
经验公式(希尔记法):
C10H14N2Na2O8 · 2H2O
CAS号:
分子量:
372.24
Beilstein:
3900609
EC 号:
PubChem化学物质编号:
NACRES:
NA.31

等级

for molecular biology

质量水平

检测方案

99.0-101.0% (titration)

形式

powder

reaction suitability

reagent type: chelator

technique(s)

electrophoresis: suitable

杂质

≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

溶解性

3  M NaOH: 1.6 g/10 mL

痕量阳离子

Fe: ≤0.005%
Pb: ≤0.002%

适用性

suitable for electrophoresis
suitable for molecular biology

异质活性

DNase, RNase, NICKase and protease, none detected

储存温度

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

正在寻找类似产品? Visit 产品对比指南

一般描述

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests

应用

EDTA disodium salt has been used in research:
  • as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
  • an anticoagulant for whole blood collection
  • a stabilizer solution for freezing cell culture samples
  • in combination with PBS and trypsin, to trypsinize cells
  • for phytoextraction of heavy metals in polluted soils
二价阳离子的螯合剂。 对需要二价阳离子发挥活性的酶,比如金属蛋白酶具有抑制作用。

特点和优势

  • Molecular Biology grade, suitable for many molecular biology applications
  • Free of endonucleases DNase, RNase, Nickase, and Protease

其他说明

For additional information on our range of Biochemicals, please complete this form.

象形图

Exclamation markHealth hazard

警示用语:

Warning

危险声明

危险分类

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

靶器官

Respiratory Tract

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

为方便起见,与您过往购买产品相关的文件已保存在文档库中。

访问文档库

难以找到您所需的产品或批次号码?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can solutions of Product E5134, EDTA disodium salt, be autoclaved?

    Yes.

  4. Is Product E5134, EDTA disodium salt, stable in solution?

    Yes. A 0.5 M stock solution at pH 8.5 is stable for months at 2-8°C.

  5. What is the working concentration for Product E5134, EDTA disodium salt, for use as an anti-coagulant?

    The optimal concentration is 1.5 mg per mL of blood.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

External quality assessment of methylmalonic acid and total homocysteine
Clinical Chemistry, 45, 1536-1542 (1999)
Expression of ABCG2 and Bmi-1 in oral potentially malignant lesions and oral squamous cell carcinoma.
Dalley AJ, et al.
Cancer Medicine, 3(2), 273-283 (2014)
Ana Amaral et al.
Animals : an open access journal from MDPI, 11(1) (2021-01-21)
Neutrophils can originate neutrophil extracellular traps (NETs). Myeloperoxidase (MPO) is a peroxidase found in NETs associated to equine endometrosis and can be inhibited by 4-aminobenzoic acid hydrazide (ABAH). Metallopeptidases (MMPs) participate in extracellular matrix stability and fibrosis development. The objectives
External quality assessment of methylmalonic acid and total homocysteine.
M?ller J, et al.
Clinical Chemistry, 45(9), 1536-1542 (1999)
Chelant-enhanced heavy metal uptake by Eucalyptus trees under controlled deficit irrigation
Fine P et al.
The Science of the Total Environment, 493, 995-1005 (2014)

实验方案

This protocol describes a method for chemical cross-linking of proteins using formaldehyde. With the exception of zero-length cross-linkers, formaldehyde has the shortest cross-linking span (~2-3 Å) of any cross-linking reagent, thus making it an ideal tool for detecting specific protein-protein interactions with great confidence.

本协议描述了一种使用甲醛完成化学交联蛋白质的方法。除零长交联剂外,甲醛是交联跨度最短(~2-3 Å)的交联剂,因此使其成为非常可靠地检测特定蛋白质-蛋白质相互作用的理想工具。

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门