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Merck
CN

E5134

乙二胺四乙酸 二钠盐 二水合物

suitable for electrophoresis, Molecular Biology, 99.0-101.0% (titration)

别名:

Sequestrene Na₂, EDTA 二钠盐, EDTA-Na2, 乙二胺四乙酸Na2, 乙二胺四乙酸盐 二钠盐 二水合物, 二钠乙二胺四乙酸盐 二水合物, 依地酸钙钠

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关于此项目

经验公式(希尔记法):
C10H14N2Na2O8 · 2H2O
化学文摘社编号:
分子量:
372.24
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12352107
EC Number:
205-358-3
Beilstein/REAXYS Number:
3900609
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grade

Molecular Biology

Quality Level

assay

99.0-101.0% (titration)

form

powder

reaction suitability

reagent type: chelator

technique(s)

electrophoresis: suitable

impurities

≤0.005% Insolubles, ≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

solubility

3  M NaOH: 1.6 g/10 mL

cation traces

Fe: ≤0.005%, Pb: ≤0.002%

suitability

suitable for electrophoresis, suitable for molecular biology

foreign activity

DNase, RNase, NICKase and protease, none detected

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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General description

脱水EDTA二钠盐可螯合金属离子,与二价阳离子形成八面体络合物。在分子生物学研究中可用于抑制依赖金属离子激活的酶。在诊断检测中,可作为采血抗凝剂用于血液学检验。

Application

EDTA二钠盐在研究中的应用:
  • 作为电泳、细胞培养、酶法检测、siRNA、流式细胞术、二甲基亚甲蓝等的缓冲液组分
  • 作为全血采集的抗凝剂
  • 作为细胞培养物样本冷冻的稳定剂
  • 与PBS和胰蛋白酶共同用于消化细胞
  • 用于污染土壤的重金属植物萃取
二价阳离子的螯合剂。 对需要二价阳离子发挥活性的酶,比如金属蛋白酶具有抑制作用。

Features and Benefits

  • 分子生物学级,适于许多分子生物学应用
  • 不含内切核酸酶DNA酶、RNA酶、切口酶和蛋白酶

pictograms

Exclamation markHealth hazard

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

target_organs

Respiratory Tract

存储类别

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

External quality assessment of methylmalonic acid and total homocysteine.
M?ller J, et al.
Clinical Chemistry, 45(9), 1536-1542 (1999)
Ana Amaral et al.
Animals : an open access journal from MDPI, 11(1) (2021-01-21)
Neutrophils can originate neutrophil extracellular traps (NETs). Myeloperoxidase (MPO) is a peroxidase found in NETs associated to equine endometrosis and can be inhibited by 4-aminobenzoic acid hydrazide (ABAH). Metallopeptidases (MMPs) participate in extracellular matrix stability and fibrosis development. The objectives
Chelant-enhanced heavy metal uptake by Eucalyptus trees under controlled deficit irrigation
Fine P et al.
The Science of the Total Environment, 493, 995-1005 (2014)
External quality assessment of methylmalonic acid and total homocysteine
Clinical Chemistry, 45, 1536-1542 (1999)
Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.

实验方案

Formaldehyde cross-linking protocol detects specific protein interactions with confidence.

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

本协议描述了一种使用甲醛完成化学交联蛋白质的方法。除零长交联剂外,甲醛是交联跨度最短(~2-3 Å)的交联剂,因此使其成为非常可靠地检测特定蛋白质-蛋白质相互作用的理想工具。

全球贸易项目编号

货号GTIN
E5134-100G04061835544509
E5134-5KG04061835544547
E5134-50G04061835544530
E5134-250G04061835544523
E5134-1KG04061835544516
E5134-500G04061838357540

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