生物来源
human leucocytes
质量水平
表单
lyophilized powder
比活
≥50 units/mg protein (Bradford)
分子量
29 kDa
UniProt登记号
运输
dry ice
储存温度
−20°C
基因信息
human ... ELA2(1991), ELANE(1991)
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一般描述
弹性蛋白酶是一种蛋白水解酶，属于肽基肽水解酶亚组成员。它是动脉的主要解剖组分。主要存在于各种鸟类和哺乳动物的胰腺和胰液中，还存在于人血清、粒细胞和红细胞中。
白细胞弹性蛋白酶是蛋白酶 S1 家族的一个 29 kDa 的丝氨酸内切蛋白酶。以单个 238 个氨基酸-肽链形式存在，有 4 个二硫键。其含有两种或多种组成的 N-连接聚糖，这解释了其三种主要亚型。
等电点：pI = 8.77-9.55
等电点：pI = 8.77-9.55
应用
弹性蛋白酶（来源于人白细胞）已用于：
- 测定血清弹性蛋白酶活性
- 纤连蛋白和唾液腺的蛋白水解消化
- 中性粒细胞弹性蛋白酶(NE)活性测定
- E-钙粘蛋白的无细胞中性粒细胞弹性蛋白酶消化
- 细胞划痕实验
- 研究确定Nle3-血管紧张素(1-7)片段可加速皮肤模型的愈合
生化/生理作用
弹性蛋白酶在蛋白水解过程的辅助下可从不溶性弹性蛋白纤维中释放可溶性多肽。它在腺苷三磷酸(ATP)的辅助下可激活轴丝解体。与胰腺弹性蛋白酶不同，白细胞酶优先切割缬氨酸的羧基侧，一定几率下也会在丙氨酸基团后进行切割。天然底物包括弹性蛋白、软骨蛋白聚糖、I、II、II和IV型胶原以及纤连蛋白。
外形
从 0.05 M 乙酸钠 (pH 5.5) 和 0.6 MNaCl 冻干
其他说明
37°C，pH 6.5 下，1 个单位每秒钟将从 BOC- L -丙氨酸 4-硝基苯酯中释放 1 纳摩尔4-硝基苯酚。
储存分类代码
13 - Non Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
高风险级别生物产品--人源产品
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