产品名称
Anti-< EDEM1 兔抗, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 65 kDa (nonglycosylated human and mouse EDEM1)
antigen 75 kDa
species reactivity
human, mouse
technique(s)
immunoprecipitation (IP): 0.5-1.0 μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein.
western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... EDEM1(9695)
mouse ... Pvalb(19293)
General description
Anti-EDEM1 recognizes glycoslylated human and mouse EDEM1 with molecular weight 75 kDa and nonglycosylated human and mouse EDEM1 with a molecular
weight 65 kDa.
weight 65 kDa.
ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1) is an enzyme encoded by the EDEM1 gene in humans. In mammalian cells, EDEM1 is localized to the endoplasmic reticulum (ER), mainly as a soluble glycoprotein.
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Application
Anti-EDEM1 antibody has been used in immunoblotting.
Anti-EDEM1 antibody produced in rabbit is suitable for the following applications:
- Immunoprecipitation at a concentration of 0.5-1.0μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein.
- Western blotting at a concentration of 0.5-1.0μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1.
- Immunofluorescence Microscopy.
- Immunoblotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1), a putative mannose binding lectin, targets misfolded glycoproteins for degradation in an N-glycan dependent manner. It interacts with the C-terminus of calnexin and lacks mannosidase activity.
ER degradation-enhancing alpha-mannosidase-like 1 is an enzyme encoded by the EDEM1 gene in humans. It is a chaperone for rod opsin and its expression can be used to promote correct folding enhancing degradation of mutant proteins in the ER to combat protein-misfolding disease. The endogenous EDEM1 in cells that are not stressed by the expression of a transgenic misfolded protein reaches the cytosol and is degraded by basal autophagy. EDEM may maintain the retrotranslocation competence of NHK by inhibiting aggregation. It is also considered responsible for directing aberrant proteins for ERAD (ER-associated protein degradation).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
synthetic peptide corresponding to amino acids 629-646 of human EDEM1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence is identical in mouse EDEM1.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Sujin Park et al.
Histochemistry and cell biology, 142(2), 153-169 (2014-03-26)
ER degradation-enhancing α-mannosidase-like 1 protein (EDEM1) is involved in the routing of misfolded glycoproteins for degradation in the cytoplasm. Previously, we reported that EDEM1 leaves the endoplasmic reticulum via non-COPII vesicles (Zuber et al. in Proc Natl Acad Sci USA
EDEM1 recognition and delivery of misfolded proteins to the SEL1L-containing ERAD complex
Cormier J H, et al.
Molecular Cell, 34(5), 627-633 (2009)
Redundant and antagonistic roles of XTP3B and OS9 in decoding glycan and non-glycan degrons in ER-associated degradation
van der Goot A T, et al.
Molecular Cell, 70, 516-530 (2018)
Iryna Monastyrska et al.
Autophagy, 9(2), 164-174 (2012-11-28)
Equine arteritis virus (EAV) is an enveloped, positive-strand RNA virus. Genome replication of EAV has been associated with modified intracellular membranes that are shaped into double-membrane vesicles (DMVs). We showed by immuno-electron microscopy that the DMVs induced in EAV-infected cells
Fulvio Reggiori et al.
Cell host & microbe, 7(6), 500-508 (2010-06-15)
Coronaviruses (CoV), including SARS and mouse hepatitis virus (MHV), are enveloped RNA viruses that induce formation of double-membrane vesicles (DMVs) and target their replication and transcription complexes (RTCs) on the DMV-limiting membranes. The DMV biogenesis has been connected with the
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