assay
>95%
form
powder
mol wt
~800,000 Da
color
orange
solubility
H2O: soluble 1 mg/mL
storage temp.
2-8°C
Quality Level
Application
荧光素透明质酸已用于透明质酸 (ha) 和葡聚糖表面涂层膜 和图案化表面的荧光成像。在共聚焦荧光显微镜中也用于定位胶原/HA 复合凝胶中的透明质酸。
Biochem/physiol Actions
利用与荧光素胺偶联的透明质酸大分子 (HA) 通过荧光相关光谱法 (FCS) 通过测量荧光素标记的 HA 片段的猝灭来筛选透明质酸酶活性。它结合在源自人肿瘤的恶性间皮瘤的细胞表面上。
用于与人类肿瘤来源的恶性间皮瘤细胞表面结合。
Other Notes
来自鸡冠或通过发酵。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Hyaluronan receptors are expressed on human malignant mesothelioma cells but not on normal mesothelial cells
Asplund T and Heldin P
Cancer research, 54(16), 4516-4523 (1994)
T Asplund et al.
Cancer research, 54(16), 4516-4523 (1994-08-15)
Hyaluronan-binding sites were demonstrated on the cell surface of three malignant mesothelioma cell lines derived from human tumors using either [3H]hyaluronan or fluorescein-tagged hyaluronan. No hyaluronan-binding activity was observed on normal human mesothelial cells. The absence of hyaluronan receptors on
Rafal Fudala et al.
Journal of photochemistry and photobiology. B, Biology, 106, 69-73 (2011-11-16)
In this report we propose a lifetime-based sensing (LBS) for the detection of hyaluronidase (HA-ase). First, we heavily label hyaluronan macromolecules (HAs) with fluorescein amine. The fluorescein labeled HA (HA-Fl) has a weak fluorescence and short fluorescence lifetime due to
Hyaluronan and dextran modified tubes resist cellular activation with blood contact
Eckmann DM, et al.
Colloids and Surfaces. B, Biointerfaces, 108, 44-51 (2013)
Rheology and confocal reflectance microscopy as probes of mechanical properties and structure during collagen and collagen/hyaluronan self-assembly
Yang Yl and Kaufman LJ
Biophysical Journal, 96(4), 1566-1585 (2009)
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