F3022
抗-β-肌动蛋白–FITC抗体，小鼠单克隆
clone AC-15, purified from hybridoma cell culture
别名:
单克隆抗-β-Actin
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选择尺寸
关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
AC-15, monoclonal
Application:
direct immunofluorescence
Species reactivity:
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
Citations:
23
Technique(s):
direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts
Uniprot accession no.:
产品名称
抗-β-肌动蛋白–FITC抗体，小鼠单克隆, clone AC-15, purified from hybridoma cell culture
biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AC-15, monoclonal
form
buffered aqueous solution
mol wt
antigen 42 kDa
species reactivity
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
should not react with
Dictyostelium discoideum
technique(s)
direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
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Application
单克隆小鼠抗肌动蛋白抗体被用作大鼠肝脏蛋白裂解液western blot分析的一种上样对照。
单克隆小鼠抗肌动蛋白抗体被用作一种上样对照，用于对来自大鼠背根神经节共培养物的免疫沉淀蛋白进行western blot分析。
小鼠抗 β-肌动蛋白-FITC 单克隆抗体可用于：
- 蛋白质印迹
- 免疫组化
- 流式细胞术
- 直接免疫荧光染色
- 双重免疫荧光染色
Biochem/physiol Actions
β-肌动蛋白调节细胞生长、迁移和 G-肌动蛋白库。ACTB变异导致多效性发育障碍。
肌动蛋白主要与细胞运动有关。它普遍存在于细胞质中，并参与无数的细胞过程，包括运动、分泌、细胞质流动、吞噬作用和胞质分裂。它在哺乳动物和鸟类中表达，并且已经鉴定出至少六种亚型。
肌动蛋白是一种真核蛋白。它属于主要的细胞骨架蛋白家族，主要与细胞运动有关。它普遍存在于细胞质中，并参与无数的细胞过程，包括运动、分泌、细胞质流动、吞噬作用和胞质分裂。它在哺乳动物和鸟类中表达，并且已经鉴定出至少六种亚型。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
单克隆抗-β-肌动蛋白（小鼠 IgG1 亚型）抗体来自于免疫小鼠骨髓瘤细胞和脾细胞相互融合而产生的 AC-15 杂交瘤细胞。ACTB（肌动蛋白 β）基因负责编码 β-肌动蛋白。它是一种细胞骨架管家蛋白。存在于细胞质中。该基因位于人染色体7p22.1。 肌动蛋白是一种真核蛋白。它属于主要的细胞骨架蛋白家族。
在鸡砂囊超薄组织冷冻切片的染色中，抗体对致密体和由结蛋白和膜相关的致密斑所占据的纵向通道连接的连续致密体进行了标记。它不会对成人的心脏和骨骼肌进行染色，除非是受到了非肌肉细胞样本的污染，或是使用了胚胎组织。
Immunogen
略微修改的β-细胞质肌动蛋白N末端肽，Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys，偶联至KLH。
Other Notes
想要查看肌动蛋白抗体选择指南，请访问 www.sigmaaldrich.com/actin。
Physical form
溶于0.01M磷酸盐缓冲盐水，pH 7.4，含有1%牛血清白蛋白以及15 mM叠氮化钠。
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Rajeevkumar R Nair et al.
Frontiers in synaptic neuroscience, 9, 8-8 (2017-05-30)
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Decreased expression of angiogenesis antagonist EFEMP1 in sporadic breast cancer is caused by aberrant promoter methylation and points to an impact of EFEMP1 as molecular biomarker
Sadr NA, et al.
International Journal of Cancer. Journal International Du Cancer, 124(7), 1727-1735 (2009)
CD9 regulates transcription factor GCM1 and ERVWE1 expression through the cAMP/protein kinase A signaling pathway
Muroi Y, et al.
Reproduction (Cambridge, England), 138(6), 945-951 (2009)
Zurab Siprashvili et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 9(5), 721-728 (2004-05-04)
Despite numerous attractive intracellular targets, protein therapeutics have been principally confined to the extracellular space due to the lack of a straightforward way to deliver functional polypeptides to the cell interior. Peptide sequences facilitating intracellular protein delivery have been identified;
Shuan Rao et al.
Nature communications, 5, 3056-3056 (2014-01-22)
Autophagy is a mechanism by which starving cells can control their energy requirements and metabolic states, thus facilitating the survival of cells in stressful environments, in particular in the pathogenesis of cancer. Here we report that tissue-specific inactivation of Atg5
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