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Merck
CN

F3046

单克隆抗肌动蛋白抗体

clone AC-40, purified immunoglobulin, buffered aqueous solution

别名:

Monoclonal Anti-Actin

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203

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产品名称

单克隆抗肌动蛋白抗体, clone AC-40, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-40, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat

technique(s)

direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... ACTA1(58), ACTA1(58), ACTA1(58), ACTA2(59), ACTA2(59), ACTA2(59), ACTB(60), ACTB(60), ACTB(60), ACTC1(70), ACTC1(70), ACTC1(70), ACTG1(71), ACTG1(71), ACTG1(71), ACTG2(72), ACTG2(72), ACTG2(72)
mouse ... Acta1(11459), Acta1(11459), Acta1(11459), Acta2(11475), Acta2(11475), Acta2(11475), Actb(11461), Actb(11461), Actb(11461), Actc1(11464), Actc1(11464), Actc1(11464), Actg1(11465), Actg1(11465), Actg1(11465), Actg2(11468), Actg2(11468), Actg2(11468)
rat ... Acta1(29437), Acta1(29437), Acta1(29437), Acta2(81633), Acta2(81633), Acta2(81633), Actb(81822), Actb(81822), Actb(81822), Actc1(29275), Actc1(29275), Actc1(29275), Actg1(287876), Actg1(287876), Actg1(287876), Actg2(25365), Actg2(25365), Actg2(25365)

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Application

肌动蛋白单克隆抗体被用于Cos-7细胞裂解物的蛋白质免疫印迹分析，以确保等量蛋白质上样。
已使用肌动蛋白单克隆抗体作为一抗，对MDCK细胞裂解物进行蛋白质免疫印迹分析。
Actin–FITC antibody is suitable for direct immunofluorescence at a dilution of 1:75, using cultured human or chicken fibroblasts.

General description

该抗体可识别在所有肌动蛋白亚型中均保守的C末端表位。它能够特异性标记各种组织和物种中的肌动蛋白。 被抗体识别的表位能够耐受甲醛固定和石蜡包埋。锌-福尔马林、B5、乙醇、methacarn、Brunnel's或Bouin's溶液也可用作固定液。
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. It has one N-terminal region which appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

Immunogen

与多抗原肽（MAP）骨架连接的合成肌动蛋白C末端肽

Biochem/physiol Actions

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. It is associated with myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. In mammals and birds, actin is expressed as six different isoforms. It has one N-terminal region which appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

含少量动物源组分生物产品
常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000475633
0000475633
0000159059
0000186492
0000186492

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K Sutoh et al.
Biochemistry, 25(20), 6186-6192 (1986-10-07)
An antibody was raised against the N-terminal 18 residues of rabbit skeletal muscle actin. By the use of this antibody as the N-terminal probe of actin and the fluorescent label at Cys-374 as its C-terminal probe, binding sites of depactin
J L Lessard
Cell motility and the cytoskeleton, 10(3), 349-362 (1988-01-01)
Two IgG1, kappa monoclonal antibodies (Mab) against actin have been obtained from a fusion in which chicken gizzard actin was used as the immunogen. One Mab, designated B4, shows a preferential reactivity toward enteric smooth muscle actin but also cross-reacts
Kimberly A Moore et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 232(2), 268-281 (2004-12-23)
Local alterations in the mechanical compliance of the basement membrane that alter the level of isometric tension in the cell have been postulated to influence tissue morphogenesis. To explore whether cell tension contributes to tissue pattern formation in vivo, we
J S Drew et al.
The American journal of physiology, 260(6 Pt 1), C1332-C1340 (1991-06-01)
Smooth muscle cells contain both smooth muscle-specific and cytoplasmic actin isoforms. We tested the hypothesis that these two classes of isoactins are segregated into separate "contractile" vs. "cytoskeletal" populations of thin filaments. Isolated thin filaments from adult swine stomach were
The actin gene family: function follows isoform.
Perrin B J and Ervasti J M
Cytoskeleton (Hoboken, N.J.), 67(10), 630-634 (2010)
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Data Sheet - F3046

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