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生物来源
Escherichia coli (O111:B4)
质量水平
偶联物
FITC conjugate
形式
lyophilized powder
标记范围
2-10 μg FITC per mg LPS
颜色
yellow to orange
溶解性
H2O: soluble 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow
储存温度
2-8°C
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应用
FITC-脂多糖（FITC-LPS）可用于研究LPS跨组织屏障（如结肠上皮细胞）的运动/转运和细胞结合以及脂多糖与荧光测定系统的内化等过程。它可用于评估、鉴定和定量脂多糖（LPS）去除试剂和流程。FITC-LPS偶联物可用于检测LPA结合蛋白和基序。
脂多糖（LPS）是革兰氏阴性菌细胞壁的特征组分。LPS及其脂质A部分可通过能够识别常见的病原体相关分子模式（PAMP）的Toll样受体蛋白家族成员Toll样受体4（TLR4）刺激先天性免疫系统细胞。
生化/生理作用
脂多糖（LPS）位于膜的外层，并且暴露在无包膜菌株的细胞表面。它们有助于维持外层膜的完整性，并保护细胞免受胆汁盐和亲脂性抗生素的破坏。
制备说明
本品可溶于水(5 mg/ml)，得到浑浊的黄色至橙色溶液。脂多糖分子可在各种溶剂中形成胶束。在水和磷酸盐缓冲盐水中，可观察到混浊的溶液。使用有机溶剂不会得到更澄清的溶液。以甲醇为溶剂将得到含漂浮物的混浊悬浮液，而以水为溶剂将得到均匀的混浊溶液。 用于细胞培养时，应通过向小瓶（1mg）中加入1ml无菌平衡盐溶液或细胞培养基重悬LPS，并轻轻旋转直至粉末溶解。可通过另外添加无菌平衡盐溶液或细胞培养基，将溶液进一步稀释至所需的工作浓度。
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产品编号
说明
价格
警示用语：
Danger
危险声明
危险分类
Acute Tox. 2 Oral
储存分类代码
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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