F4125
无花果蛋白酶 来源于无花果树乳胶
saline suspension, ≥1.0 units/mg protein (biuret)
别名:
Debricin, Ficain, higueroxyl delabarre
登录 查看组织和合同定价。
选择尺寸
关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥1.0 units/mg protein (biuret)
Biological source:
fig tree (latex)
产品名称
无花果蛋白酶 来源于无花果树乳胶, saline suspension, ≥1.0 units/mg protein (biuret)
biological source
fig tree (latex)
form
saline suspension
specific activity
≥1.0 units/mg protein (biuret)
mol wt
23.8 kDa
storage temp.
2-8°C
Quality Level
正在寻找类似产品? 访问 产品对比指南
Biochem/physiol Actions
无花果蛋白酶（Ficin）被归类为硫醇蛋白酶。它的活性位点上含有单个反应性半胱氨酸。其活性位点的氨基酸同源性与木瓜蛋白酶相似。无花果蛋白酶将切割Gly、Ser、Thr、Met、Lys、Arg、Tyr、Ala、Asn和Val羧基侧的蛋白质。据报道，显色底物pGlu-Phe-Leu-p-硝基苯胺的 Km 为0.43 mM。无花果蛋白酶可被碘乙酰胺、碘乙酸、N-乙基马来酰亚胺、氯化汞、DFP（二异丙基氟磷酸酯）、TLCK（Na-对甲苯磺酰基-赖氨酸氯甲基酮）和TPCK（N-甲苯磺酰基-L-苯丙氨酸氯甲基酮）抑制。无花果蛋白酶可用于以高得率产生小鼠IgG1的F (ab′)2 片段。
General description
消光系数： E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.
Other Notes
在 pH 7.0 和 37°C 下，当在最终体积为 10mL（1cm 光程）的溶液中测定酪蛋白的 TCA 可溶性产物时，一单位每分钟产生的 ΔA280 为 1.0。
Physical form
2.0M NaCl 和 0.03M 半胱氨酸中的悬浮液 (pH 5.0)
Preparation Note
2 次结晶
Application
Ficin from fig tree latex has been used:
- in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality
- for the digestion of eye tissue sections prior to immunostaining and immunofluorescence
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
动植物源性产品
此项目有
Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex
Zare H, et al.
Phytochemistry, 87, 16-22 (2013)
Transgenic expression of leukemia inhibitory factor (LIF) blocks normal vascular development but not pathological neovascularization in the eye
Ash J, et al.
Molecular Vision, 11, 298-308 (2005)
Yufang Yang et al.
Scientific reports, 7, 43141-43141 (2017-02-23)
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for
Lens-specific VEGF-A expression induces angioblast migration and proliferation and stimulates angiogenic remodeling
Ash JD and Overbeek PA
Developmental Biology, 223(2), 383-398 (2000)
E Saitoh et al.
Archives of biochemistry and biophysics, 352(2), 199-206 (1998-05-20)
Two variants of cystatin SA encoded by two alleles at the CST2 locus of the type 2 human cystatin gene family were expressed in Escherichia coli. One, termed cystatin SA1, is identical to cystatin SA [S. Isemura, E. Saitoh, and
实验方案
This procedure may be used for all Ficin products.
相关内容
Product Information Sheet
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持