F4165
无花果蛋白酶 来源于无花果树乳胶
powder, ≥0.1 unit/mg solid
别名:
Debricin, Ficain, higueroxyl delabarre
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-599-1
MDL number:
Specific activity:
≥0.1 unit/mg solid
Biological source:
fig tree (latex)
产品名称
无花果蛋白酶 来源于无花果树乳胶, powder, ≥0.1 unit/mg solid
biological source
fig tree (latex)
form
powder
specific activity
≥0.1 unit/mg solid
mol wt
23.8 kDa
storage temp.
−20°C
Quality Level
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Biochem/physiol Actions
无花果蛋白酶（Ficin）被归类为硫醇蛋白酶。它的活性位点上含有单个反应性半胱氨酸。其活性位点的氨基酸同源性与木瓜蛋白酶相似。无花果蛋白酶将切割Gly、Ser、Thr、Met、Lys、Arg、Tyr、Ala、Asn和Val羧基侧的蛋白质。据报道，显色底物pGlu-Phe-Leu-p-硝基苯胺的 Km 为0.43 mM。无花果蛋白酶可被碘乙酰胺、碘乙酸、N-乙基马来酰亚胺、氯化汞、DFP（二异丙基氟磷酸酯）、TLCK（Na-对甲苯磺酰基-赖氨酸氯甲基酮）和TPCK（N-甲苯磺酰基-L-苯丙氨酸氯甲基酮）抑制。无花果蛋白酶可用于以高得率产生小鼠IgG1的F (ab′)2 片段。
General description
消光系数： E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
Other Notes
一个酶活性单位是指在测量酪蛋白的TCA可溶性产物时，在终体积为10ml（1cm光路）、37°C、pH 7.0条件下每分钟产生1.0的ΔA280 所需的酶量。
该酶溶于1 M磷酸钾缓冲液中，pH 7.0 (0.25 mg/ml)，得到澄清溶液。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
此项目有
Yufang Yang et al.
Scientific reports, 7, 43141-43141 (2017-02-23)
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for
Andrey V Sorokin et al.
Polymers, 14(11) (2022-06-11)
This work aims to synthesize graft copolymers of chitosan and N-vinylimidazole (VI) with different compositions to be used as matrices for the immobilization of cysteine proteases-bromelain, ficin, and papain. The copolymers are synthesized by free radical solution copolymerization with a
Kamsagara Basavarajappa Devaraj et al.
Journal of agricultural and food chemistry, 56(23), 11417-11423 (2008-11-11)
Ficin (EC 3.4.22.3), a cysteine proteinase isolated from the latex of a Ficus tree, is known to occur in multiple forms. Although crude ficin is of considerable commercial importance, ficin as such has not been fully characterized. A major ficin
Daniel Janvier et al.
Transfusion, 53(9), 1948-1955 (2012-12-18)
Rh proteins and the Wr(b) antigen, which results from an interaction between Band 3 and glycophorin A, are the most common targets for warm immunoglobulin (Ig)G autoantibodies. Apart from autoanti-Di(b) , a scarce specificity, IgG warm autoantibodies specific for Band
S Fadýloğlu
Die Nahrung, 45(2), 143-146 (2001-05-31)
Fig tree latex (ficin) was immobilized on Celite by adsorption. The free and immobilized ficin were utilized in the production of teleme (a Turkish milk product). After immobilization, the optimal temperature of ficin was shifted from 60 to 80 degrees
实验方案
This procedure may be used for all Ficin products.
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