F4165
无花果蛋白酶 来源于无花果树乳胶
powder, ≥0.1 unit/mg solid
别名:
Debricin, Ficain, higueroxyl delabarre
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-599-1
MDL number:
Specific activity:
≥0.1 unit/mg solid
Biological source:
fig tree (latex)
biological source
fig tree (latex)
Quality Level
form
powder
specific activity
≥0.1 unit/mg solid
mol wt
23.8 kDa
storage temp.
−20°C
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General description
消光系数： E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
Biochem/physiol Actions
无花果蛋白酶（Ficin）被归类为硫醇蛋白酶。它的活性位点上含有单个反应性半胱氨酸。其活性位点的氨基酸同源性与木瓜蛋白酶相似。无花果蛋白酶将切割Gly、Ser、Thr、Met、Lys、Arg、Tyr、Ala、Asn和Val羧基侧的蛋白质。据报道，显色底物pGlu-Phe-Leu-p-硝基苯胺的 Km 为0.43 mM。无花果蛋白酶可被碘乙酰胺、碘乙酸、N-乙基马来酰亚胺、氯化汞、DFP（二异丙基氟磷酸酯）、TLCK（Na-对甲苯磺酰基-赖氨酸氯甲基酮）和TPCK（N-甲苯磺酰基-L-苯丙氨酸氯甲基酮）抑制。无花果蛋白酶可用于以高得率产生小鼠IgG1的F (ab′)2 片段。
Other Notes
一个酶活性单位是指在测量酪蛋白的TCA可溶性产物时，在终体积为10ml（1cm光路）、37°C、pH 7.0条件下每分钟产生1.0的ΔA280 所需的酶量。
该酶溶于1 M磷酸钾缓冲液中，pH 7.0 (0.25 mg/ml)，得到澄清溶液。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
低风险生物材料
此项目有
Yufang Yang et al.
Scientific reports, 7, 43141-43141 (2017-02-23)
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for
E Saitoh et al.
Archives of biochemistry and biophysics, 352(2), 199-206 (1998-05-20)
Two variants of cystatin SA encoded by two alleles at the CST2 locus of the type 2 human cystatin gene family were expressed in Escherichia coli. One, termed cystatin SA1, is identical to cystatin SA [S. Isemura, E. Saitoh, and
Kristien Bonroy et al.
Journal of immunological methods, 312(1-2), 167-181 (2006-05-06)
The sensitivity of immunosensors is strongly dependent on the amount of immobilised antibodies and their remaining antigen binding properties. The use of smaller and well-oriented antibody fragments as bioreceptor molecules influences the final immunosensor signal. The aim of this study
H Sekizaki et al.
Amino acids, 34(1), 149-153 (2007-07-10)
The capability of ficin, a cystine protease, to form peptide bonds was investigated using several types of N-Boc-amino acid phenyl and naphthyl esters as acyl donor components. Enzyme-catalyzed peptide synthesis was carried out under optimized reaction conditions of pH, acyl
Eiichi Saitoh et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 141(1), 103-109 (2005-04-12)
A novel cysteine protease inhibitor (Eel-CPI-1) was isolated from the epidermis of the eel. Eel-CPI-1 was shown to bind strongly to both lactose- and carboxymethylated papain-affinity gels. Its molecular mass under reducing condition was determined to be 18 kDa by
实验方案
This procedure may be used for all Ficin products.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| F4165-1KU | 04061833617564 |
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