F4274
荧光素异硫氰酸酯异构体I
≥97.5% purity (HPLC), powder
别名:
FITC, 荧光素5-异硫氰酸酯
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关于此项目
经验公式（希尔记法）:
C21H11NO5S
化学文摘社编号:
分子量:
389.38
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
222-042-0
MDL number:
Beilstein/REAXYS Number:
1407295
产品名称
荧光素异硫氰酸酯异构体I, ≥97.5% (HPLC)
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
assay
≥97.5% (HPLC)
form
powder
technique(s)
titration: suitable
color
yellow to very dark yellow
mp
>360 °C (lit.)
solubility
acetone: soluble 10 mg/mL
fluorescence
λex 492 nm; λem 518 nm (green)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
Quality Level
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Application
用于蛋白FITC标记的试剂；蛋白和肽的微测序（HPLC）
荧光素异硫氰酸酯异构体 I已用于分生孢子的染色。
General description
异硫氰酸荧光素（FITC）广泛用于通过胺基将荧光标记附着到蛋白质上。异硫氰酸酯基团与蛋白质中的氨基末端和伯胺反应。它已用于标记蛋白质，包括抗体和凝集素。
Packaging
无底玻璃瓶。内容在插入的熔锥内。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
PU.1 serves a critical role in the innate defense against Aspergillus fumigatus via dendritic cell-associated C-type lectin receptor-1 and toll-like receptors-2 and 4 in THP-1-derived macrophages.
Liu C
Molecular Medicine Reports, 15, 4084-4084 (2017)
Handbook of Affinity Chromatography (2005)
Ana Bratuša et al.
Colloids and surfaces. B, Biointerfaces, 181, 561-566 (2019-06-12)
This work describes the derivatization of dextran using N-(tert-butyloxycarbonyl)-S-(trityl)-L-cysteine in the presence of N,N'-carbonyldiimidazole (CDI) as a coupling agent. Homogeneous reactions in dimethyl sulfoxide allowed for an efficient coupling of the amino acid derivative to the polymer backbone. Derivatization was
Binding of live conidia of Aspergillus fumigatus activates in vitro-generated human Langerhans cells via a lectin of galactomannan specificity.
Persat F
Clinical and Experimental Immunology, 133, 370-370 (2003)
Hermanson GT
Bioconjugate Techniques (2013)
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