F4274
荧光素异硫氰酸酯异构体I
≥97.5% purity (HPLC), powder
别名:
FITC, 荧光素5-异硫氰酸酯
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关于此项目
经验公式（希尔记法）:
C21H11NO5S
化学文摘社编号:
分子量:
389.38
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
222-042-0
MDL number:
Beilstein/REAXYS Number:
1407295
产品名称
荧光素异硫氰酸酯异构体I, ≥97.5% (HPLC)
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
assay
≥97.5% (HPLC)
form
powder
technique(s)
titration: suitable
color
yellow to very dark yellow
mp
>360 °C (lit.)
solubility
acetone: soluble 10 mg/mL
fluorescence
λex 492 nm; λem 518 nm (green)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
Quality Level
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Application
用于蛋白FITC标记的试剂；蛋白和肽的微测序（HPLC）
荧光素异硫氰酸酯异构体 I已用于分生孢子的染色。
General description
异硫氰酸荧光素（FITC）广泛用于通过胺基将荧光标记附着到蛋白质上。异硫氰酸酯基团与蛋白质中的氨基末端和伯胺反应。它已用于标记蛋白质，包括抗体和凝集素。
Packaging
无底玻璃瓶。内容在插入的熔锥内。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
PU.1 serves a critical role in the innate defense against Aspergillus fumigatus via dendritic cell-associated C-type lectin receptor-1 and toll-like receptors-2 and 4 in THP-1-derived macrophages.
Liu C
Molecular Medicine Reports, 15, 4084-4084 (2017)
Binding of live conidia of Aspergillus fumigatus activates in vitro-generated human Langerhans cells via a lectin of galactomannan specificity.
Persat F
Clinical and Experimental Immunology, 133, 370-370 (2003)
Handbook of Affinity Chromatography (2005)
Ana Bratuša et al.
Colloids and surfaces. B, Biointerfaces, 181, 561-566 (2019-06-12)
This work describes the derivatization of dextran using N-(tert-butyloxycarbonyl)-S-(trityl)-L-cysteine in the presence of N,N'-carbonyldiimidazole (CDI) as a coupling agent. Homogeneous reactions in dimethyl sulfoxide allowed for an efficient coupling of the amino acid derivative to the polymer backbone. Derivatization was
Hermanson GT
Bioconjugate Techniques (2013)
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