biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
RG-16, monoclonal
form
buffered aqueous solution
should not react with
pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
storage condition
protect from light
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
Application
Monoclonal Anti-Rabbit Immunoglobulins-FITC antibody produced in mouse has been used in:
- immunocytochemistry
- immunohistochemistry
- immunofluorescence
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.
Biochem/physiol Actions
Binds only rabbit Igs. Does not react with reduced rabbit Igs.
Physical form
Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
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