一般描述
10% Neutral buffered formalin (NBF) is the most commonly used fixative for light microscopy. 10% NBF is well suited for large throughput laboratories and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.
应用
Formalin, 10%, Neutral Buffered, Wintergreen Scented has been used as a fixative in transformation and tumorigenicity assay and in immunohistostaining.
Intended for use as a general purpose histological fixative. Wintergreen oil has been added to the standard formulation to provide a wintergreen scent.
警示用语：
Danger
危险分类
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1
储存分类代码
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Donnenberg VS, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 83(1), 141-149 (2013)
A method to estimate the formaldehyde concentration in fixative solutions
Simos, P
Pathology, 43, 394-394 (2001)
Effects of formalin fixation on tissue optical
M F G Wood
Physics in Medicine and Biology, 56, V115-V115 (2011)
p38alpha MAP kinase as a sensor of reactive oxygen species in tumorigenesis
Dolado I, et al.
Cancer Cell, 11(2), 191-205 (2007)
Neither epithelial nor mesenchymal circulating tumor cells isolated from breast cancer patients are tumorigenic in NOD-scid Il2rg null mice
Donnenberg VS, et al.
NPJ breast cancer, 2, 16004-16004 (2016)
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