生物来源
mouse
质量水平
偶联物
FITC conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
W6/32, monoclonal
表单
buffered aqueous solution
种属反应性
monkey, human
技术
direct immunofluorescence: 1:10 using acetone-fixed human tonsil frozen sections.
flow cytometry: 10 μL using 1 × 106 cells
同位素/亚型
IgG2a
UniProt登记号
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... HLA-A(3105), HLA-B(3106), HLA-C(3107)
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一般描述
Monoclonal Anti-Human HLA Class I Antigen (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from BALB/c mice immunized with human tonsil leukocyte membrane preparation. Class I proteins are highly polymorphic. HLA-A, -B, -C are classical class I proteins and are heterodimers. HLA-A consists of a polymorphic transmembrane α heavy chain and an invariant water-soluble light chain.
The function of HLA class I molecules is to present antigen to CD8+ lymphocytes. They are involved in the regulation of positive and negative selection of CD8+ T cells by thymic epithelial cells during T cell maturation. These molecules are important in allograft rejection and in the linkage of certain haplotypes in autoimmune diseases. Expression of HLA class I antigens may be modulated in pathological states. Malignant cells may lose these antigens while hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may display enhanced reactivity. Increased expression of these antigens is described in muscle cells affected by muscular dystrophy, inflammatory myopathy and other neuro-muscular disorders.
The previously assigned protein identifier P01892 has been merged into P04439. Full details can be found on the UniProt database.
The previously assigned protein identifier P01892 has been merged into P04439. Full details can be found on the UniProt database.
特异性
识别大多数人类有核细胞上表达的人类HLA I类抗原。抗体与人I类HLA分子(HLA-A,B,C)的44-45 kDa α-链多肽上的单态表位反应。该抗体可用于丙酮固定的冰冻切片和细胞涂片的免疫沉淀、补体介导细胞毒性分析、流式细胞分析和免疫组化染色。其可与非洲和亚洲猿猴以及旧大陆猴的细胞发生交叉反应。其与大多数新大陆猴或非灵长类动物的细胞均未见反应性。该抗体识别的表位对常规福尔马林固定和石蜡包埋敏感。
第 4 次研讨会:代码P1
第 5 次研讨会:代码 BP166、BP288、BP407、B 005
第 4 次研讨会:代码P1
第 5 次研讨会:代码 BP166、BP288、BP407、B 005
免疫原
人扁桃体白细胞膜制备。
应用
FITC Monoclonal Anti-Human HLA Class I Antigen may be used for immunochemical analysis of HLA Class I antigens; Studies of HLA-A,B,C expression on embryonic and neoplastic tissues; and/or the purification of HLA-A,B,C antigens.
Monoclonal Anti-HLA Class I Antigen-FITC antibody produced in mouse has been used in:
- flow cytometric analysis
- immunoprecipitation
- complement mediated cytotoxicity
- immunocytochemistry
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
制备说明
Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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