F6008
无花果蛋白酶 来源于无花果树乳胶
lyophilized powder
别名:
Debricin, Ficain, higueroxyl delabarre
登录 查看组织和合同定价。
选择尺寸
关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-599-1
MDL number:
Specific activity:
≥1.0 units/mg protein
Biological source:
fig tree (latex)
产品名称
无花果蛋白酶 来源于无花果树乳胶, lyophilized powder
biological source
fig tree (latex)
form
lyophilized powder
specific activity
≥1.0 units/mg protein
mol wt
23.8 kDa
composition
Protein, 80-100% biuret
storage temp.
−20°C
Quality Level
正在寻找类似产品? 访问 产品对比指南
Physical form
冻干粉含有 L-半胱氨酸和微量EDTA
General description
消光系数： E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
Preparation Note
该酶可溶于水（10 mg/ml），并产生澄清溶液。
Other Notes
在pH 7.0，37°C条件下，一个单位每分钟可产生ΔA280，该活性是在终体积为10 ml的反应体系中对来源于酪蛋白的TCA可溶性产物进行测定（1 cm 光路）。
Application
来自无花果树乳胶的无花果蛋白酶已被用于:
- 肌腱胶原蛋白的蛋白水解
- 石莼属绿藻的消化
- 研究其激活蛋白酶激活受体 2和 4 诱发皮肤瘙痒的作用
Biochem/physiol Actions
无花果蛋白酶可以有效地分解葡萄球菌生物膜，从而使其接触抗生素。
无花果蛋白酶（Ficin）被归类为硫醇蛋白酶。它的活性位点上含有单个反应性半胱氨酸。其活性位点的氨基酸同源性与木瓜蛋白酶相似。无花果蛋白酶将切割Gly、Ser、Thr、Met、Lys、Arg、Tyr、Ala、Asn和Val羧基侧的蛋白质。据报道，显色底物pGlu-Phe-Leu-p-硝基苯胺的 Km 为0.43 mM。无花果蛋白酶可被碘乙酰胺、碘乙酸、N-乙基马来酰亚胺、氯化汞、DFP（二异丙基氟磷酸酯）、TLCK（Na-对甲苯磺酰基-赖氨酸氯甲基酮）和TPCK（N-甲苯磺酰基-L-苯丙氨酸氯甲基酮）抑制。无花果蛋白酶可用于以高得率产生小鼠IgG1的F (ab′)2 片段。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
此项目有
Targeting microbial biofilms using Ficin, a nonspecific plant protease
Baidamshina DR, et al.
Scientific reports, 7, 46068-46068 (2017)
Generation of an artificial skin construct containing a non-degradable fiber mesh: a potential transcutaneous interface
Cahn F and Kyriakides TR
Biomedical Materials (Bristol, England), 3(3), 034110-034110 (2008)
Yusei Takemoto et al.
Journal of cellular physiology, 234(2), 1689-1698 (2018-08-03)
Nuclear factor erythroid 2-related factor 2 (Nrf2) is a master regulator for the induction of antioxidative genes and plays roles in diverse cellular functions. The roles of Nrf2 in muscle regeneration have been investigated, and both important and unimportant roles
Isolation and characterization of angiotensin I-converting enzyme (ACE) inhibitory peptides from Ulva rigida C. Agardh protein hydrolysate
Paiva L, et al.
Journal of functional foods, 26, 65-76 (2016)
A Aktypis et al.
Journal of applied microbiology, 84(4), 568-576 (1998-06-20)
ACA-DC 0040 produced an antimicrobial agent, which was named thermophilin T, active against several lactic acid bacteria strains of different species and food spoilage bacteria, such as Clostridium sporogenes C22/10 and Cl. tyrobutyricum NCDO-1754. The crude antimicrobial compound is sensitive
实验方案
This procedure may be used for all Ficin products.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持