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Merck
CN

F6057

Fluoroshield 封固剂(含DAPI)

liquid

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关于此项目

UNSPSC Code:
12171500
NACRES:
NA.47
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产品名称

Fluoroshield 封固剂(含DAPI), liquid

description

histology mounting medium

Quality Level

form

liquid

composition

Sodium Azide, ≤1% (also contains other ingredients), Tris-HCl

technique(s)

immunofluorescence: suitable

pH range

7.9-8.3

solubility

water: soluble

fluorescence

λex 360 nm; λem 460 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

General description

含DAPI的Fluoroshield是一种含有4′,6-二氨基-2-苯基吲哚(DAPI)的水固载介质,也是一种核反染剂,可用作蓝色荧光DNA探针。它是一种抗褪色剂,用于保存组织和细胞涂片的荧光,通过防止快速光漂白和最小化眨眼介导的现象。(1)用于原位杂交技术或其他需要DNA染色荧光的方法。DAPI的激发波长为360nm,发射波长为460nm,产生蓝色荧光。RNA也可以采用DAPI染色。

Application

Fluoroshield封固剂(含DAPI)可用于 :
  • 用作免疫细胞化学中的复染剂,可标记神经祖细胞的细胞核
  • 用作免疫荧光研究中心脏组织部分的组织学封固剂
  • 用作细胞核检测中免疫组化染色心脏部分的封固溶液

Legal Information

FluoroShield is a trademark of ImmunoBioScience Corp.

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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S Hamada et al.
Histochemistry, 79(2), 219-226 (1983-01-01)
DNA-DAPI complexes emit strong bluish white fluorescence when excited by ultraviolet light so that even very small amounts of DNA such as those in mitochondria, chloroplasts, and virus particles can be visualized. Moreover, the staining procedure with DAPI is very
miR-210 suppresses BNIP3 to protect against the apoptosis of neural progenitor cells
Wang F, et al.
Stem Cell Research, 11(1), 657-667 (2013)
Human umbilical cord tissue-derived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms
Nascimento DS, et al.
Stem Cell Research & Therapy, 5(1), 5-5 (2014)
Extracellular matrix-derived extracellular vesicles promote cardiomyocyte growth and electrical activity in engineered cardiac atria
An M, et al.
Biomaterials, 146(1), 49-59 (2017)
Edgar T Hoorntje et al.
PloS one, 13(8), e0203078-e0203078 (2018-08-31)
Likely pathogenic/pathogenic variants in genes encoding desmosomal proteins play an important role in the pathophysiology of arrhythmogenic right ventricular cardiomyopathy (ARVC). However, for a substantial proportion of ARVC patients, the genetic substrate remains unknown. We hypothesized that plectin, a cytolinker

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全球贸易项目编号

货号GTIN
F6057-20ML04061833618448

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