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Merck
CN

F6182

Sigma-Aldrich

Fluoroshield

liquid

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About This Item

UNSPSC代码:
12171500
NACRES:
NA.47

product name

Fluoroshield, histology mounting medium

质量水平

形式

liquid

技术

immunofluorescence: suitable

pH值范围

7.8-8.2

溶解性

water: soluble

应用

diagnostic assay manufacturing
hematology
histology

储存温度

2-8°C

一般描述

Fluoroshield封固剂是一种水性封固剂,用于保持组织和细胞涂片的荧光。这种独特的配方不仅可以延长荧光时间,还可防止FITC、Texas Red®、AMCA、 Cy®、Cy3、Cy5、Alexa Fluor 488、Alexa Fluor 594、绿色荧光蛋白(GFP)、四甲基罗丹明、R-藻红蛋白(R-PE)、藻蓝蛋白(PC)和别藻蓝蛋白(APC)等荧光染料的快速光漂白效应(褪色)。

应用

Fluoroshield 用于:
  • 作为经培养的巨核细胞和免疫染色的血小板的固封剂,用于共聚焦显微镜研究
  • 作为4′,6-二氨基-2-苯基吲哚(DAPI)染色的心脏切片的固封剂,用于免疫荧光研究
  • 保持C6细胞涂片的荧光

联系

Fluoroshield 是一种与凝胶支架相当的水性封片剂

法律信息

ALEXA FLUOR is a trademark of Life Technologies
Cy is a registered trademark of Cytiva
Cy3 is a trademark of Cytiva
FluoroShield is a trademark of ImmunoBioScience Corp.
Gel Mount is a trademark of Biomeda Corp.
Texas Red is a registered trademark of Life Technologies

相关产品

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. How do I store Fluoroshield (Product Nos. F6182, F5932, F6057) ?

    This product is stored at 2-8°C.  Protect from light, and do not freeze.

  7. How do I use Fluoroshield (Product Nos. F6182, F5932, F6057)?

    1. Bring the vial to room temperature. 2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, then touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light. 3. Turn the vial upside down and remove any air bubbles. 4. Apply 3-4 drops directly of mounting medium on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip, making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide on paper towel. 5. Let stand at room temperature for about 5 minutes. 6. Apply cover slip, carefully avoiding air bubbles. 7. The specimen is ready for visualization under microscope. 8. One can seal the edges of cover slip with nail polish or any organic medium. If coverslip is not used air bubbles will appear in few days. 9. For long term storage it is recommended that the slide be stored in the dark at 2-8°C.

  8. How do you remove the coverslip when using Fluoroshield (Product Nos. F6182, F5932, F6057)?

    The coverslip can be removed before sealing the edges. Soak slide in warm (37  °C) water for a few minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.

  9. What fluorochromes can be used with Fluoroshield (Product Nos. F6182, F5932, F6057)?

    This unique formula prevents rapid photobleaching of FITC, Texas Red®, AMCA, Cy®2, Cy®3, Cy®5, Alexa Fluor® 488, Alexa Fluor® 594, green fluorescent protein (GFP), tetramethylrhodamine, R-phycoerythrin (R-PE), phycocyanin (PC), and allophycocyanin (APC).  Fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of the Cy dyes, RP-E, PC and APC.

  10. Does Fluoroshield (Product Nos. F6182, F5932, F6057) contain an antifade reagent?

    This unique formula prevents rapid photobleaching.  The exact nature of the antifade is considered proprietary.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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