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F7250

荧光素异硫氰酸酯异构体I

Name: 荧光素异硫氰酸酯异构体I
Brand: SIGMA

≥90% purity (HPLC), powder

别名:

FITC, 荧光素5-异硫氰酸酯

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关于此项目

经验公式（希尔记法）:

C₂₁H₁₁NO₅S

化学文摘社编号:

3326-32-7

分子量:

389.38

NACRES:

NA.47

PubChem Substance ID:

24894933

UNSPSC Code:

12171500

EC Number:

222-042-0

MDL number:

MFCD00005063

Beilstein/REAXYS Number:

1407295

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产品名称

荧光素异硫氰酸酯异构体I, suitable for protein labeling, ≥90% (HPLC), powder

Quality Level

200

assay

≥90% (HPLC)

form

powder

technique(s)

titration: suitable

color

orange to dark orange

mp

>360 °C (lit.)

solubility

acetone: 1 mg/mL

fluorescence

λ_ex 492 nm; λ_em 518 nm (green)

suitability

suitable for protein labeling

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

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General description

荧光素异硫氰酸酯（FITC）具有黄橘色的颜色以及在495 nm处的最大吸光值。通过激发，它可以散发出在525 nm具有最大发射的黄绿色。
它被广泛用于通过胺基将荧光标记附着到蛋白质上。异硫氰酸酯基团可与蛋白中的氨基末端和伯胺反应。它已用于对蛋白质标记，包括抗体和凝集素。
异硫氰酸荧光素异构体I已被提议作为接触敏化剂。

Application

用于蛋白FITC标记的试剂；蛋白和肽的微测序（HPLC）
生物学应用，包括用于蛋白荧光标记试剂、用于蛋白示踪的荧光试剂以及作为病原体快速鉴定的荧光抗体技术中的一种试剂。在毛细管电泳（CE）测定过程中，它可用作人尿液中苯丙胺、甲基苯丙胺、3,4-亚甲二氧基甲基苯丙胺和对苯乙胺的衍生试剂。它可用于荧光抗体的制备。它可用于体外敏化研究。

用于蛋白FITC标记的试剂；蛋白和肽的微测序（HPLC）

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Analysis of fluorescein isothiocyanate derivatized amphetamine and analogs in human urine by capillary electrophoresis in chip-based and fused-silica capillary instrumentation.

A Ramseier et al.

Electrophoresis, 19(16-17), 2967-2975 (1998-12-31)

Amines can easily be derivatized with fluorescein isothiocyanate isomer I (FITC) and analyzed by capillary electrophoresis (CE) using alkaline buffers with or without dodecyl sulfate micelles. This paper reports the CE analysis of FITC-derivatized amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and beta-phenylethylamine in

Dendritic cells and the initiation of contact sensitivity to fluorescein isothiocyanate.

S E Macatonia et al.

Immunology, 59(4), 509-514 (1986-12-01)

Lymph node cells taken 24 hr after skin-painting mice with the contact sensitizer fluorescein isothiocyanate (FITC) induce delayed-type hypersensitivity in recipient mice. Skin-painting increased the number of dendritic cells (DC) in the draining lymph nodes without significantly changing the number

Chromatographically pure fluorescein and tetramethylrhodamine isothiocyanates.

L C FELTON et al.

Analytical biochemistry, 2, 178-180 (1961-04-01)

Test of a two-pathway model for small-solute exchange across the capillary wall.

Fu BM

The American Journal of Physiology, 274, H2062-H2062 (1998)

Effects of thermized donkey milk with lysozyme activity on altered gut barrier in mice exposed to water-avoidance stress.

Sophie Yvon et al.

Journal of dairy science, 102(9), 7697-7706 (2019-07-22)

Nutrition plays a crucial role in human gut health through the improvement of gut barrier functionality. Donkey milk represents an interesting source of natural antimicrobial factors such as lysozyme. Recently, anti-inflammatory properties of donkey milk lysozyme activity were described in

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全球贸易项目编号

货号	GTIN
F7250-250MG	04061835573011
F7250-500MG	04061835573028
F7250-5G	04061833619346
F7250-1G	04061835506323
F7250-50MG	04061833619339
F7250-100MG	04061835512546

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