FD40S
异硫氰酸荧光素-葡聚糖
average mol wt 40,000
别名:
FITC-右旋糖酐
biological source
synthetic (organic)
conjugate
FITC conjugate
form
powder
mol wt
average mol wt 40,000
extent of labeling
0.003-0.020 mol FITC per mol glucose
color
yellow to dark orange
solubility
H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange
storage temp.
2-8°C
Quality Level
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General description
葡聚糖是葡萄糖酐聚合物。由大约95%的α-D-(166)键组成。剩余的（163）键作为葡聚糖的分支。 天然葡聚糖的分子量(MW)经证实在 900 万至 5 亿的范围内。FITC-葡聚糖的最大激发波长为 490nm。它的 pH 范围为 5 至 9。
Application
异硫氰酸荧光素-葡聚糖（FITC-右旋糖酐，荧光素右旋糖酐，FITC）用于以下过程：
- 荧光素-葡聚糖显微镜。
- 评估多形核白细胞α防御素表达转基因小鼠体内肺血管的通透性。
- 通过流式细胞术定量细胞吞噬能力。
- 标记α乳白蛋白和脱氧核糖核酸酶。
用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。
Preparation Note
Sigma 通常以 25mg/ml 或更高的浓度测试 FITC 葡聚糖在水中的溶解度。 溶液应避光。 在体内，FITC-葡聚糖稳定超过 24 小时。
Other Notes
与 FD-40 类似，但由 Sigma 生产。
天然葡聚糖的分子量(MW)经证实在 900 万至 5 亿的范围内。较低分子量的葡聚糖将表现出略微较少的分支，并且具有更窄的分子量分布范围。分子量大于 10,000 的葡聚糖则表现为高度支化。 随着分子量增加，葡聚糖分子的对称性增强。 分子量为 2,000 至 10,000 的葡聚糖表现出可膨胀线圈的特性。 在分子量低于 2,000 时，葡聚糖更像棒状。通过以下一种或多种方法测量葡聚糖的分子量：低角度激光散射、尺寸排阻色谱法、铜络合和蒽酮试剂显色还原末端糖测定和粘度。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Here we present a simple technique for re-directing reactions on the cell surface to the outermost region of the glycocalyx. Macromolecular crowding with inert polymers was utilized to reversibly alter the accessibility of glycocalyx proteoglycans toward cell-surface reactive probes allowing
Molecular weight, molecular-weight distribution and molecular size of native dextran.
AROND L H and H P FRANK
The Journal of Physical Chemistry, 953 - 957 (1954)
相关内容
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.
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