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Merck
CN

G1041

Sigma-Aldrich

EZBlue ™ 凝胶染色试剂

别名:

protein gel stain, protein stain

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UNSPSC代码:
41105300
NACRES:
NA.32

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技术

protein staining: suitable

质量水平

200

储存温度

2-8°C

相关类别

一般描述

EZBlue ™ 考马斯亮蓝 G-250 胶体蛋白染色方便、灵敏、安全，在显著减少染色时间的同时，改善了蛋白电泳结果。EZBlue包装方便， 不需要忙乱的称重或添加甲醇或酸。作为胶体染色剂，它只与蛋白质反应，而不与凝胶本身反应。背景染色减少，因此蛋白条带几乎可以立即显示出来。无需脱色步骤，但水洗可能会强化条带并澄清背景。最令人印象深刻的是，EZBlue 极为灵敏，可检测低至 5 ng 的蛋白质。

应用

EZBlue™凝胶染色试剂已被用作十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)中的染色试剂。

特点和优势

  • 预混溶液 省去了制备染色剂所需的时间和精力
  • 灵敏度的提高 确保低丰度蛋白（低至 5 ng）可被检测到
  • 快速反应 显著减少了染色和冲洗所需的时间
  • 无溶剂废物 ，因此可以通过消除有害物质处置步骤来节省时间和金钱

其他说明

即用型亮蓝 G-250 基蛋白染色剂，用于聚丙烯酰胺凝胶和 PVDF 膜上的“一步”超灵敏检测。

法律信息

EZBlue is a trademark of Sigma-Aldrich Co. LLC

象形图

Health hazardCorrosion
GHS08,GHS05

警示用语：

Warning

危险分类

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

靶器官

Eyes,Central nervous system

储存分类代码

8A - Combustible corrosive hazardous materials

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000358257
0000358257
0000358259
0000358259
SLCN0167

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商品

Cell Lysate Preparation

Cell lysis challenges and protein extraction variables for Western blot sensitivity and reproducibility.

Western Blot Analysis of Immunoprecipitation (IP-Western)

The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

Rapid Glycan Prep & Analysis by LC-MS Workflow

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

蛋白电泳染色剂选择指南

为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。

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实验方案

使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌制PAGE和SDS-PAGE凝胶

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

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