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Merck
CN

G1544

抗-GFP,N端 兔抗

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

抗-绿色荧光蛋白

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
300
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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates, western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

绿色荧光蛋白(GFP)生色团含具有Phe64-Ser-Tyr-Gly-Val-Gln69氨基酸序列的六肽片段。GFP结构包含11链β-桶状结构和中心螺旋,对应分子量为26 kDa。

Immunogen

合成多肽对应GFP氨基酸3-17。

Application

兔抗GFP(N末端抗体)抗体用于:
  • 蛋白质印迹
  • 组织化学和免疫组化
  • 免疫沉淀


Biochem/physiol Actions

对于GFP融合蛋白有特异性。
绿色荧光蛋白(GFP)应用于光化学和诊断领域。也广泛应用于蛋白动力学研究。多克隆抗-GFP、兔抗N端抗体可与GFP融合蛋白发生特异性反应。

Physical form

溶于0.01M磷酸盐缓冲盐水,pH 7.4,含有1%牛血清白蛋白以及15 mM叠氮化钠。

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
低风险生物材料

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros
Liu Y, et al.
Development, 134(6), 1111-1122 (2007)
Green fluorescent protein: a perspective
Remington SJ
Protein Science, 20(9), 1509-1519 (2011)
A phosphorylation-wide sncRNA screen reveals Protein Functional Effector sncRNAs (pfeRNAs) in human lung somatic cells
Gable T, et al.
Cancer letters, 396(6), 85-93 (2017)
Lingfeng Meng et al.
Cell reports, 11(11), 1737-1748 (2015-06-16)
Synapse elimination occurs in development, plasticity, and disease. Although the importance of synapse elimination has been documented in many studies, the molecular mechanisms underlying this process are unclear. Here, using the development of C. elegans RME neurons as a model
Pathogenic mechanism of recurrent mutations of SCN8A in epileptic encephalopathy
Wagnon JL, et al.
Annals of Clinical and Translational Neurology, 3(2), 114-123 (2016)

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全球贸易项目编号

货号GTIN
G1544-100UG04061833628270

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