产品名称
抗-GFP，N端 兔抗, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates
western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Application
兔抗GFP（N末端抗体）抗体用于：
- 蛋白质印迹
- 组织化学和免疫组化
- 免疫沉淀
Biochem/physiol Actions
对于GFP融合蛋白有特异性。
绿色荧光蛋白（GFP）应用于光化学和诊断领域。也广泛应用于蛋白动力学研究。多克隆抗-GFP、兔抗N端抗体可与GFP融合蛋白发生特异性反应。
General description
绿色荧光蛋白（GFP）生色团含具有Phe64-Ser-Tyr-Gly-Val-Gln69氨基酸序列的六肽片段。GFP结构包含11链β-桶状结构和中心螺旋，对应分子量为26 kDa。
Immunogen
合成多肽对应GFP氨基酸3-17。
Physical form
溶于0.01M磷酸盐缓冲盐水，pH 7.4，含有1%牛血清白蛋白以及15 mM叠氮化钠。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
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