等级
Molecular Biology
质量水平
表单
liquid
包装
vial of 1 mL
浓度
19-22 mg/mL
储存温度
−20°C
SMILES字符串
O1[C@@H]([C@@H]([C@H]([C@@H]([C@H]1CO[C@H]4O[C@@H]([C@H]([C@@H]([C@H]4O)O)O)CO)O[C@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O)CO)O)O)O[C@@H]2[C@H](O[C@@H]([C@@H]([C@H]2O)O)O)CO
InChI
1S/C24H42O21/c25-1-5-9(28)11(30)16(35)22(41-5)39-4-8-20(45-23-17(36)12(31)10(29)6(2-26)42-23)14(33)18(37)24(43-8)44-19-7(3-27)40-21(38)15(34)13(19)32/h5-38H,1-4H2/t5-,6-,7-,8-,9-,10-,11+,12+,13-,14-,15-,16-,17-,18-,19-,20-,21+,22+,23-,24-/m1/s1
InChI key
BYSGBSNPRWKUQH-UJDJLXLFSA-N
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一般描述
Glycogen is a branched polymer of glucose synthesized by animal cells for energy storage and release. It is constructed of predominantly α1→4 glycosidic bonds with branches created through α1→6 glycosidic bonds.
Glycogen is a branched polymer of glucose that coprecipitates nucleic acids during isolation and purification.
应用
Glycogen from Mytilus genus (Blue mussel) has been used
- as a coprecipitant in the isolation of RNA from hepatocytes and yeast cells.
- as a reference standard in calibration curve generation to quantify hepatic glycogen content.
- as a carrier molecule for DNA and RNA, replacing tRNA or sonicated DNA
- for the precipitation of DNA in the presence of ammonium acetate and ethanol or isopropanol
- for recovery of tRNA, yeast RNA, sonicated DNA and oligonucleotides as short as 8 base pairs
生化/生理作用
Glycogen is an inert carrier that traps nucleic acids. It forms a precipitate with DNA/RNA while being insoluble in ethanol solution. Due to the manufacturing process, there is low risk of contaminating nucleic acids in the glycogen. This product is tested for DNase, RNase, and NICKase, but not nucleic acids.
Glycogen levels in Mytilus genus shows variation during seasonal changes. It is a crucial stored energy reserve for Mussels and is utilized during gametogenesis. Glycogen levels dictate the reproductive capacity and development of mussels larva. Glycogen aids in the coprecipitation of nucleic acids during isolation and purification.
特点和优势
- product provided is 1 mL total volume
- precipitates even small amounts of DNA and RNA present in dilute solutions
- does not interfere with OD 260/280 readings
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Regulation of gluconeogenesis by Kruppel-like factor 15
Gray S, et al.
Cell Metabolism, 5(4), 305-312 (2007)
Energy storage and reproduction in mussels, Mytilus galloprovincialis: the influence of diet quality
Fearman JA, et al.
Journal of shellfish research., 28(2), 305-312 (2009)
Tri-methylation of histone H3 lysine 4 facilitates gene expression in ageing cells
Cruz C, et al.
eLife, 7, e34081-e34081 (2018)
Mitchell B Rosen et al.
Toxicology, 308, 129-137 (2013-04-10)
While perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) have been studied at length, less is known about the biological activity of other perfluoroalkyl acids (PFAAs) detected in the environment. Using a transient transfection assay developed in COS-1 cells, our group
Tue Fryland et al.
Genome medicine, 8(1), 53-53 (2016-05-05)
The bromodomain containing 1 (BRD1) gene has been implicated with transcriptional regulation, brain development, and susceptibility to schizophrenia and bipolar disorder. To advance the understanding of BRD1 and its role in mental disorders, we characterized the protein and chromatin interactions
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