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Merck
CN

G4018

Sigma-Aldrich

Anti-Goat IgG (whole molecule) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

rabbit

质量水平

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

secondary antibodies

克隆

polyclonal

表单

buffered aqueous solution

技术

indirect ELISA: 1:60,000
quantitative precipitin assay: 2.0 mg/mL

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

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一般描述

IgG is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.
The product binds to all goat Igs.

免疫原

Goat IgG purified from a pool of normal goat serum.

应用

Anti-Goat IgG (whole molecule) antibody has been used in immunohistochemistry and in lateral flow strips (LF- strips).
Anti-Goat IgG (whole molecule) antibody produced in rabbit was used as control antibody in shear stress experiments conducted on porcine endothelial cells. It was used in ChIP assay to study the H19 imprinting control region in transgenic Drosophila.

生化/生理作用

IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.

制备说明

Anti-Goat IgG (whole molecule)is an affinity isolated polyclonal antibody developed in rabbit using goat IgG purified from pooled goat serum as the immunogen. The antibody is purified to remove rabbit serum proteins including immunoglobulins which do not specifically bind to goat IgG.

分析说明

Each milliliter of product contains 2.0-2.5 mg of specific antibody measured by quantitative precipitation. Identity and purity is determined by immunoelectrophoresis(IEP).

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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