G5420
抗-β-葡萄糖醛酸酶（N-末端） 兔抗
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
抗-GUS
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 60 kDa
species reactivity
plant
concentration
~1.5 mg/mL
technique(s)
western blot: 0.5-1 μg/mL using GUS purified from E. coli
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
β-葡萄糖醛酸酶(GUS)由大肠杆菌GUS基因（也称为uidA）编码，GUS蛋白(60 kDa)是一种水解酶。
Immunogen
与位于大肠杆菌GUS的N-末端的氨基酸相对应的合成肽，与KLH偶联。
Application
兔多克隆抗-β-葡糖醛酸糖苷酶（N-末端）抗体可用于通过免疫印迹（60kDa）检测 GUS。 它可用于检测转基因植物中的报告分子 GUS标记蛋白。
已在免疫印迹中使用兔体抗-β-葡萄糖醛酸酶（N-端）抗体。
Biochem/physiol Actions
β-葡萄糖醛酸酶(GUS)是一种筛选标记，大肠杆菌GUS已被广泛用于监测转基因向植物组织的转运。
β-葡萄糖醛酸酶催化通过β-键与单糖、寡糖或多糖或酚类相连的末端葡萄糖醛酸的断裂反应。GUS主要用作转基因事件期间的标记。体外GUS活性测定主要促进组织化学和在组织荧光定位。GUS活性也可用于外源基因表达的分析。使用与特定启动子基因融合的GUS报告基因以在含抗生素培养基上测定转化体。GUS特定抗体对于检测转化植物中GUS基因产物特别有用。
抗-β-葡糖醛酸糖苷酶（N-末端）识别转基因烟草植物中表达的细菌GUS。
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Preparation Note
若需连续使用，可在2-8°C下保存最长一个月。若需延长储存时间，可将溶液分装并冷冻。不建议反复冻融或存放在“无霜”冰柜中。如果长期储存后发生轻微浑浊，请在使用前通过离心澄清溶液。如果在12小时内未使用，则应丢弃工作稀释样品。
Disclaimer
除非我们的产品目录或产品随附的其他公司文档中另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途，或任何类型的消费，或应用于人或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
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