biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 60 kDa
species reactivity
plant
concentration
~1.5 mg/mL
technique(s)
western blot: 0.5-1 μg/mL using GUS purified from E. coli
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
β-葡萄糖醛酸酶(GUS)由大肠杆菌GUS基因(也称为uidA)编码,GUS蛋白(60 kDa)是一种水解酶。
Immunogen
与位于大肠杆菌GUS的N-末端的氨基酸相对应的合成肽,与KLH偶联。
Application
兔多克隆抗-β-葡糖醛酸糖苷酶(N-末端)抗体可用于通过免疫印迹(60kDa)检测 GUS。 它可用于检测转基因植物中的报告分子 GUS标记蛋白。
已在免疫印迹中使用兔体抗-β-葡萄糖醛酸酶(N-端)抗体。
Biochem/physiol Actions
β-葡萄糖醛酸酶(GUS)是一种筛选标记,大肠杆菌GUS已被广泛用于监测转基因向植物组织的转运。
β-葡萄糖醛酸酶催化通过β-键与单糖、寡糖或多糖或酚类相连的末端葡萄糖醛酸的断裂反应。GUS主要用作转基因事件期间的标记。体外GUS活性测定主要促进组织化学和在组织荧光定位。GUS活性也可用于外源基因表达的分析。使用与特定启动子基因融合的GUS报告基因以在含抗生素培养基上测定转化体。GUS特定抗体对于检测转化植物中GUS基因产物特别有用。
抗-β-葡糖醛酸糖苷酶(N-末端)识别转基因烟草植物中表达的细菌GUS。
Physical form
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
Preparation Note
若需连续使用,可在2-8°C下保存最长一个月。若需延长储存时间,可将溶液分装并冷冻。不建议反复冻融或存放在“无霜”冰柜中。如果长期储存后发生轻微浑浊,请在使用前通过离心澄清溶液。如果在12小时内未使用,则应丢弃工作稀释样品。
Disclaimer
除非我们的产品目录或产品随附的其他公司文档中另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途,或任何类型的消费,或应用于人或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
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