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Merck
CN

G6378

葡萄糖-6-磷酸脱氢酶 来源于面包酵母（酿酒酵母）

Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

别名:

6-磷酸葡糖脱氢酶, G-6-P-DH

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关于此项目

化学文摘社编号:
9001-40-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
MFCD00081656
EC 号:
1.1.1.49 (BRENDA, IUBMB)

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产品名称

葡萄糖-6-磷酸脱氢酶 来源于面包酵母（酿酒酵母）, Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

type

Type XV

form

lyophilized powder

specific activity

200-400 units/mg protein (modified Warburg-Christian)

mol wt

128 kDa

purified by

crystallization

application(s)

diagnostic assay manufacturing

shipped in

dry ice

storage temp.

−20°C

Quality Level

300

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Application

葡萄糖-6-磷酸脱氢酶被用于测试玉米胚乳形成中的酮糖还原酶活性。

Biochem/physiol Actions

葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸，作为磷酸戊糖途径的第一步<<<17>>>。
葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸，作为磷酸戊糖途径的第一步。

General description

葡萄糖6-磷酸脱氢酶（G-6-P-DH）是磷酸戊糖途径第一步的关键调节酶。G-6-P-DH是分子量为128kDa（凝胶过滤）的糖蛋白 。

Other Notes

一个酶活性单位是指在pH 7.4、25°C且存在NADP的条件下，每分钟将1.0 μM的 D-葡萄糖6-磷酸氧化成6-磷酸-D-葡糖酸盐所需的酶量。

Physical form

冻干粉，基本上无硫酸盐，按干重计含有大约20% 的柠檬酸钠

Preparation Note

由 G7877 制备

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000525537
0000525537
0000518198
0000518198
0000511903

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the Km values for Product G6378, Glucose-6-phosphate Dehydrogenase?

    The Km values for glucose 6-phosphate and NADP are 2.0 x 10-5 and 2.0 x 10-6 , respectively in Tris buffer, pH 8.0 containing 10 mm magnesium chloride at 38°C.

  6. What does Type XV mean in the description of Product G6378, Glucose-6-phosphate Dehydrogenase?

    The type designation Type XV is a Sigma in-house designation and does not represent any kind of biochemical significance. It simply refers to the order of addition of the product to the catalog (i.e., first there was a type I, then a type II, etc.).

  7. What is the activity and protein content of the lot number of Product G6378, Glucose-6-phosphate Dehydrogenase, I have?

    The protein content and the specific activity of the enzyme can be found on the lot-specific certificate of analysis.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Yong Yang et al.
Frontiers in microbiology, 9, 1428-1428 (2018-07-20)
Mycobacteria spontaneously form surface-associated multicellular communities, called biofilms, which display resistance to a wide range of exogenous stresses. A causal relationship between biofilm formation and emergence of stress resistance is not known. Here, we report that activation of a nitrogen
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
Anna Leonov et al.
Oncotarget, 13, 918-943 (2022-08-09)
We propose a hypothesis of a mechanism linking cellular aging to cellular quiescence in chronologically aging budding yeast. Our hypothesis posits that this mechanism integrates four different processes, all of which are initiated after yeast cells cultured in a medium
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实验方案

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定

在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

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