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安全信息

G6378

Sigma-Aldrich

葡萄糖-6-磷酸脱氢酶 来源于面包酵母（酿酒酵母）

Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

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别名:
6-磷酸葡糖脱氢酶, G-6-P-DH
CAS号:
9001-40-5
EC 号:
1.1.1.49 (BRENDA, IUBMB)
EC 号:
232-602-6
MDL编号:
MFCD00081656
NACRES:
NA.54

类型

Type XV

质量水平

300

形式

lyophilized powder

比活

200-400 units/mg protein (modified Warburg-Christian)

分子量

128 kDa

纯化方式

crystallization

应用

diagnostic assay manufacturing

运输

dry ice

储存温度

−20°C

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一般描述

葡萄糖6-磷酸脱氢酶（G-6-P-DH）是磷酸戊糖途径第一步的关键调节酶。G-6-P-DH是分子量为128kDa（凝胶过滤）的糖蛋白 。

应用

葡萄糖-6-磷酸脱氢酶被用于测试玉米胚乳形成中的酮糖还原酶活性。

生化/生理作用

葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸，作为磷酸戊糖途径的第一步<<<17>>>。
葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸，作为磷酸戊糖途径的第一步。

单位定义

一个酶活性单位是指在pH 7.4、25°C且存在NADP的条件下，每分钟将1.0 μM的 D-葡萄糖6-磷酸氧化成6-磷酸-D-葡糖酸盐所需的酶量。

外形

冻干粉，基本上无硫酸盐，按干重计含有大约20% 的柠檬酸钠

制备说明

由 G7877 制备

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the Km values for Product G6378, Glucose-6-phosphate Dehydrogenase?

    The Km values for glucose 6-phosphate and NADP are 2.0 x 10-5 and 2.0 x 10-6 , respectively in Tris buffer, pH 8.0 containing 10 mm magnesium chloride at 38°C.

  6. What does Type XV mean in the description of Product G6378, Glucose-6-phosphate Dehydrogenase?

    The type designation Type XV is a Sigma in-house designation and does not represent any kind of biochemical significance. It simply refers to the order of addition of the product to the catalog (i.e., first there was a type I, then a type II, etc.).

  7. What is the activity and protein content of the lot number of Product G6378, Glucose-6-phosphate Dehydrogenase, I have?

    The protein content and the specific activity of the enzyme can be found on the lot-specific certificate of analysis.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
Yong Yang et al.
Frontiers in microbiology, 9, 1428-1428 (2018-07-20)
Mycobacteria spontaneously form surface-associated multicellular communities, called biofilms, which display resistance to a wide range of exogenous stresses. A causal relationship between biofilm formation and emergence of stress resistance is not known. Here, we report that activation of a nitrogen
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
Sean Rogers et al.
eLife, 10 (2021-04-08)
Eukaryotes compartmentalize metabolic pathways into sub-cellular domains, but the role of inter-organelle contacts in organizing metabolic reactions remains poorly understood. Here, we show that in response to acute glucose restriction (AGR) yeast undergo metabolic remodeling of their mevalonate pathway that
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实验方案

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定

在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

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