G6670
Anti-Growth Factor Independence-1 antibody, Mouse monoclonal
clone 2.5D.17, purified from hybridoma cell culture
别名:
Anti-GFI1, Anti-ZNF163
产品名称
Anti-Growth Factor Independence-1 antibody, Mouse monoclonal, clone 2.5D.17, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
2.5D.17, monoclonal
form
buffered aqueous solution
mol wt
antigen ~55 kDa
species reactivity
rat, mouse, human
concentration
~2 mg/mL
technique(s)
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of A549 cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GFI1(2672)
mouse ... Gfi1(14581)
rat ... Gfi1(24388)
Analysis Note
The antibody may also be used in EMSA (electrophoretic mobility shift analysis).
Application
Monoclonal Anti-Growth Factor Independence-1 (GFI1) antibody may be used in:
- immunoblotting
- immunohistochemistry
- EMSA (electrophoretic mobility shift analysis)
Biochem/physiol Actions
Growth factor independence-1 (Gfi1) plays a key role in hematopoiesis along with Gfi1B proteins. Cytokines that regulate innate immunity induce Gfi1 expression. Deficiency of this protein causes thymic and peripherial T lymphopenia and severe abnormalities in pre-T-cell development in mice. Mutations in this gene cause defects in T lymphocyte function and development in humans. Gfi1 autoregulates its expression in T lymphocytes.
The antibody epitope resides within the GFI1 SNAG transcriptional-repression domain (amino acids 7-26).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Growth Factor Independence-1 (GFI1) (mouse IgG1 isotype) is derived from the hybridoma 2.5D.17 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with rat GFI1. Growth Factor Independence 1 (GFI1) is a zinc-finger transcriptional repressor, that is located on human chromosome 1p22. Gfi1 is highly expressed in thymus while Gfi1B is the predominant factor expressed in spleen, and both are expressed in bone marrow. It has a repression domain named SNAG at the N-terminal.† The protein also has a zinc finger DNA binding domain that is homologous to the domain in Gfi1B. Both bind to same consensus DNA sequence
Immunogen
rat growth factor independence-1 (GFI1).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Chinavenmeni S Velu et al.
Blood, 113(19), 4720-4728 (2009-03-13)
The zinc finger protein growth factor independent-1 (Gfi1) is a transcriptional repressor that is critically required for normal granulocytic differentiation. GFI1 loss-of-function mutations are found in some patients with severe congenital neutropenia (SCN). The SCN-associated GFI1-mutant proteins act as dominant
Loretta L Doan et al.
Journal of immunology (Baltimore, Md. : 1950), 170(5), 2356-2366 (2003-02-21)
T cell differentiation in the thymus is dependent upon signaling through the TCR and is characterized by the resulting changes in expression patterns of CD4 and CD8 surface coreceptor molecules. Although recent studies have characterized the effects of proximal TCR
Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells
Doan LL, et al.
Nucleic Acids Research, 32(8), 2508-2519 (2004)
Prognostic significance of high GFI1 expression in AML of normal karyotype and its association with a FLT3-ITD signature
Volpe G, et al.
Scientific reports, 7(1), 11148-11148 (2017)
The downregulation of GFI1 by the EZH2-NDY1/KDM2B-JARID2 axis and by human cytomegalovirus (HCMV) associated factors allows the activation of the HCMV major IE promoter and the transition to productive infection
Sourvinos G, et al.
PLoS Pathogens, 10(5), e1004136-e1004136 (2014)
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